Fluorogenic substrates for the detection of microbial nitroreductases.

Arthur James; C. Jay; D. Monget; John Perry; J. W. Rasburn; Frances Kate Gould

doi:10.1046/j.1472-765X.2001.01021.x

Fluorogenic substrates for the detection of microbial nitroreductases.

Arthur James, C. Jay, D. Monget, John Perry, J. W. Rasburn, Frances Kate Gould

Northumbria University

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Aims: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. Methods and Results: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. Conclusions, Significance and Impact of the Study: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.

Original language	English
Pages (from-to)	403-408
Journal	Letters in Applied Microbiology
Volume	33
Issue number	6
DOIs	https://doi.org/10.1046/j.1472-765X.2001.01021.x
Publication status	Published - Dec 2001

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title = "Fluorogenic substrates for the detection of microbial nitroreductases.",

abstract = "Aims: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. Methods and Results: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. Conclusions, Significance and Impact of the Study: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.",

keywords = "microorganisms, detection",

author = "Arthur James and C. Jay and D. Monget and John Perry and Rasburn, {J. W.} and Gould, {Frances Kate}",

note = "This paper describes the synthesis of substrates designed to detect clinically important microorganisms. Prof. James designed and synthesised the substrates. This work has been patented (James, A.; Monget, D., EP 1124986) and involved collaboration with BioMerieux",

year = "2001",

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doi = "10.1046/j.1472-765X.2001.01021.x",

language = "English",

volume = "33",

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journal = "Letters in Applied Microbiology",

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T1 - Fluorogenic substrates for the detection of microbial nitroreductases.

AU - James, Arthur

AU - Jay, C.

AU - Monget, D.

AU - Perry, John

AU - Rasburn, J. W.

AU - Gould, Frances Kate

N1 - This paper describes the synthesis of substrates designed to detect clinically important microorganisms. Prof. James designed and synthesised the substrates. This work has been patented (James, A.; Monget, D., EP 1124986) and involved collaboration with BioMerieux

PY - 2001/12

Y1 - 2001/12

N2 - Aims: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. Methods and Results: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. Conclusions, Significance and Impact of the Study: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.

AB - Aims: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. Methods and Results: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. Conclusions, Significance and Impact of the Study: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.

KW - microorganisms

KW - detection

U2 - 10.1046/j.1472-765X.2001.01021.x

DO - 10.1046/j.1472-765X.2001.01021.x

M3 - Article

VL - 33

SP - 403

EP - 408

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 6

ER -

Fluorogenic substrates for the detection of microbial nitroreductases.

Abstract

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