Acoustic streaming of ink particles inside a water microdroplet generated by a surface acoustic wave(SAW) has been studied numerically using a finite volume numerical method and these results have been verified using experimental measurements. Effects of SAW excitation frequency, droplet volume, and radio-frequency (RF) power are investigated, and it has been shown that SAW excitation frequency influences the SAWattenuation length, lSAW , and hence the acoustic energy absorbed by liquid. It has also been observed that an increase of excitation frequency generally enhances the SAW streaming behavior. However, when the frequency exceeds a critical value that depends on the RF power applied to the SAW device, weaker acoustic streaming is observed resulting in less effective acoustic mixing inside the droplet. This critical value is characterised by a dimensionless ratio of droplet radius to SAWattenuation length, i.e., Rd/lSAW . With a mean value of Rd/lSAW ≈ 1, a fast and efficient mixing can be induced, even at the lowest RF power of 0.05 mW studied in this paper. On the other hand, for the Rd/lSAW ratios much larger than ∼1, significant decreases in streaming velocities were observed, resulting in a transition from regular (strong) to irregular (weak) mixing/flow. This is attributed to an increased absorption rate of acoustic wave energy that leaks into the liquid, resulting in a reduction of the acoustic energy radiated away from the SAW interaction region towards the droplet free surface. It has been demonstrated in this study that a fast and efficient mixing process with a smaller RF power could be achieved if the ratio of Rd/lSAW ≤ 1 in the SAW-droplet based microfluidics.