From cocoa waste to sustainable bioink: valorising pectin for circular economy-driven tissue engineering

Joel Girón-Hernández*, Abraham Tombe, Mufeeda Chemban Koyilot, Karen T. Salas-Calderón, Alex Charlton, Corinne Wills, Piergiorgio Gentile*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Cocoa bean production, a cornerstone of many developing economies, currently adheres to a linear economic model, giving rise to sustainability concerns. The expansion of cocoa butter and liquor consumption has resulted in the increased generation of residual biomass, constituting 60–70 % of fresh fruit. The absence of management plans for this biomass in cocoa-producing countries poses a risk of phytosanitary issues. This study proposed a circular economy approach by valorising cocoa pod husk-derived pectin for tissue engineering. The study optimised an alkaline-based protocol to extract pectin from cocoa pod husk, resulting in ∼ 20 % yield with a ∼ 47 % degree of esterification. Methacrylation transformed extracted pectin into pectin methacrylate (PECMA), confirmed by FTIR-ATR and NMR analyses. PECMA hydrogels, crosslinked with CaCO3 and UV, exhibited rapid gelation and superior water uptake properties. SEM revealed distinct morphological differences with UV exposure, showing improved interconnectivity and anisotropic porosity while the mechanical testing demonstrated enhanced compressive modulus and rheological properties with UV crosslinking. PECMA-based bioink encapsulated chondrocytes, maintaining cell viability over 6 days. This innovative bioink, derived from cocoa waste, holds promise for sustainable tissue engineering applications.

Original languageEnglish
Article number112967
Pages (from-to)1-12
Number of pages12
JournalEuropean Polymer Journal
Volume210
Early online date24 Mar 2024
DOIs
Publication statusPublished - 24 Apr 2024

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