Function of the phosphatidylinositol transfer protein gene family: is phosphatidylinositol transfer the mechanism of action?

Shamshad Cockcroft, Kathryn Garner

Research output: Contribution to journalReview articlepeer-review

29 Citations (Scopus)

Abstract

Phosphatidylinositol transfer proteins (PITPs) bind and facilitate the transport of phosphatidylinositol (PI) and phosphatidylcholine between membrane compartments. They are highly conserved proteins, are found in both unicellular and multicellular organisms, and can be present as a single domain or as part of a larger, multi-domain protein. The hallmark of PITP proteins is their ability to sequester PI in their hydrophobic pocket. Ablation or knockdown of specific isoforms in vivo has wide ranging effects such as defects in signal transduction via phospholipase C and phosphoinositide 3-kinase, membrane trafficking, stem cell viability, Drosophila phototransduction, neurite outgrowth, and cytokinesis. In this review, we identify the common mechanism underlying each of these phenotypes as the cooperation between PITP proteins and lipid kinases through the provision of PI for phosphorylation. We propose that recruitment and concentration of PITP proteins at specific membrane sites are required for PITP proteins to execute their function rather than lipid transfer.

Original languageEnglish
Pages (from-to)89-117
Number of pages29
JournalCritical Reviews in Biochemistry and Molecular Biology
Volume46
Issue number2
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

Fingerprint

Dive into the research topics of 'Function of the phosphatidylinositol transfer protein gene family: is phosphatidylinositol transfer the mechanism of action?'. Together they form a unique fingerprint.

Cite this