Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering

Hariharan Ezhilarasu; Asif Sadiq; Greeshma Ratheesh; Sreepathy Sridhar; Seeram Ramakrishna; Mohd Hasbi Ab. Rahim; Mashitah M Yusoff; Rajan Jose; Venugopal Jayarama Reddy

doi:10.2217/nnm-2018-0271

Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering

Hariharan Ezhilarasu, Asif Sadiq, Greeshma Ratheesh, Sreepathy Sridhar, Seeram Ramakrishna, Mohd Hasbi Ab. Rahim, Mashitah M Yusoff, Rajan Jose, Venugopal Jayarama Reddy

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Aim: Atherosclerosis is a common cardiovascular disease causing medical problems globally leading to coronary artery bypass surgery. The present study is to fabricate core/shell nanofibers to encapsulate VEGF for the differentiation of mesenchymal stem cells (MSCs) into smooth muscle cells to develop vascular grafts. Materials & methods: The fabricated core/shell nanofibers contained polycaprolactone/gelatin as the shell, and silk fibroin/VEGF as the core materials. Results: The results observed that the core/shell nanofibers interact to differentiate MSCs into smooth muscle cells by the expression of vascular smooth muscle cell (VSMC) contractile proteins α-actinin, myosin and F-actin. Conclusion: The functionalized polycaprolactone/gelatin/silk fibroin/VEGF (250 ng) core/shell nanofibers were fabricated for the controlled release of VEGF in a persistent manner for the differentiation of MSCs into smooth muscle cells for vascular tissue engineering.

Original language	English
Pages (from-to)	201-214
Number of pages	14
Journal	Nanomedicine
Volume	14
Issue number	2
Early online date	10 Dec 2018
DOIs	https://doi.org/10.2217/nnm-2018-0271
Publication status	Published - 1 Jan 2019

Keywords

VEGF
core/shell nanofibers
gelatin
mesenchymal stem cells
myosin
polymers
silk fibroin
smooth muscle cells
vascular tissue engineering
α-actinin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/nnm-2018-0271

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title = "Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering",

abstract = "Aim: Atherosclerosis is a common cardiovascular disease causing medical problems globally leading to coronary artery bypass surgery. The present study is to fabricate core/shell nanofibers to encapsulate VEGF for the differentiation of mesenchymal stem cells (MSCs) into smooth muscle cells to develop vascular grafts. Materials \& methods: The fabricated core/shell nanofibers contained polycaprolactone/gelatin as the shell, and silk fibroin/VEGF as the core materials. Results: The results observed that the core/shell nanofibers interact to differentiate MSCs into smooth muscle cells by the expression of vascular smooth muscle cell (VSMC) contractile proteins α-actinin, myosin and F-actin. Conclusion: The functionalized polycaprolactone/gelatin/silk fibroin/VEGF (250 ng) core/shell nanofibers were fabricated for the controlled release of VEGF in a persistent manner for the differentiation of MSCs into smooth muscle cells for vascular tissue engineering.",

keywords = "VEGF, core/shell nanofibers, gelatin, mesenchymal stem cells, myosin, polymers, silk fibroin, smooth muscle cells, vascular tissue engineering, α-actinin",

author = "Hariharan Ezhilarasu and Asif Sadiq and Greeshma Ratheesh and Sreepathy Sridhar and Seeram Ramakrishna and \{Ab. Rahim\}, \{Mohd Hasbi\} and Yusoff, \{Mashitah M\} and Rajan Jose and Reddy, \{Venugopal Jayarama\}",

year = "2019",

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day = "1",

doi = "10.2217/nnm-2018-0271",

language = "English",

volume = "14",

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TY - JOUR

T1 - Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering

AU - Ezhilarasu, Hariharan

AU - Sadiq, Asif

AU - Ratheesh, Greeshma

AU - Sridhar, Sreepathy

AU - Ramakrishna, Seeram

AU - Ab. Rahim, Mohd Hasbi

AU - Yusoff, Mashitah M

AU - Jose, Rajan

AU - Reddy, Venugopal Jayarama

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Aim: Atherosclerosis is a common cardiovascular disease causing medical problems globally leading to coronary artery bypass surgery. The present study is to fabricate core/shell nanofibers to encapsulate VEGF for the differentiation of mesenchymal stem cells (MSCs) into smooth muscle cells to develop vascular grafts. Materials & methods: The fabricated core/shell nanofibers contained polycaprolactone/gelatin as the shell, and silk fibroin/VEGF as the core materials. Results: The results observed that the core/shell nanofibers interact to differentiate MSCs into smooth muscle cells by the expression of vascular smooth muscle cell (VSMC) contractile proteins α-actinin, myosin and F-actin. Conclusion: The functionalized polycaprolactone/gelatin/silk fibroin/VEGF (250 ng) core/shell nanofibers were fabricated for the controlled release of VEGF in a persistent manner for the differentiation of MSCs into smooth muscle cells for vascular tissue engineering.

AB - Aim: Atherosclerosis is a common cardiovascular disease causing medical problems globally leading to coronary artery bypass surgery. The present study is to fabricate core/shell nanofibers to encapsulate VEGF for the differentiation of mesenchymal stem cells (MSCs) into smooth muscle cells to develop vascular grafts. Materials & methods: The fabricated core/shell nanofibers contained polycaprolactone/gelatin as the shell, and silk fibroin/VEGF as the core materials. Results: The results observed that the core/shell nanofibers interact to differentiate MSCs into smooth muscle cells by the expression of vascular smooth muscle cell (VSMC) contractile proteins α-actinin, myosin and F-actin. Conclusion: The functionalized polycaprolactone/gelatin/silk fibroin/VEGF (250 ng) core/shell nanofibers were fabricated for the controlled release of VEGF in a persistent manner for the differentiation of MSCs into smooth muscle cells for vascular tissue engineering.

KW - VEGF

KW - core/shell nanofibers

KW - gelatin

KW - mesenchymal stem cells

KW - myosin

KW - polymers

KW - silk fibroin

KW - smooth muscle cells

KW - vascular tissue engineering

KW - α-actinin

UR - https://www.scopus.com/pages/publications/85059420580

U2 - 10.2217/nnm-2018-0271

DO - 10.2217/nnm-2018-0271

M3 - Article

SN - 1743-5889

VL - 14

SP - 201

EP - 214

JO - Nanomedicine

JF - Nanomedicine

IS - 2

ER -

Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering

Abstract

Keywords

UN SDGs

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