Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis

Elham Esmaeilishirazifard; Daniela De Vizio; Sterghios Moschos; Tajali Keshavarz

doi:10.1186/s13568-017-0381-6

Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis

Elham Esmaeilishirazifard, Daniela De Vizio, Sterghios Moschos, Tajali Keshavarz

Applied Sciences Department

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Abstract

Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS. The results obtained from bioinformatics studies on the whole genome sequence of this strain confirmed the presence of essential quorum sensing-related genes. Although polymorphism was verified in three proteins of this cluster, ComQ, precursor-ComX and ComP, the transcription factor ComA was confirmed as the most conserved protein. The cell–cell communication of B. licheniformis NCIMB-8874 was investigated through further elucidation of the ComX pheromone as 13-amino acid peptide. The peptide sequence of the pheromone has been described through biochemical characterisation.

Original language	English
Journal	AMB Express
Volume	7
Issue number	78
DOIs	https://doi.org/10.1186/s13568-017-0381-6
Publication status	Published - 8 Apr 2017

Keywords

Quorum sensing
pheromone
bacillus
gene cloning
peptide purification
genome sequencing

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title = "Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis",

abstract = "Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS. The results obtained from bioinformatics studies on the whole genome sequence of this strain confirmed the presence of essential quorum sensing-related genes. Although polymorphism was verified in three proteins of this cluster, ComQ, precursor-ComX and ComP, the transcription factor ComA was confirmed as the most conserved protein. The cell–cell communication of B. licheniformis NCIMB-8874 was investigated through further elucidation of the ComX pheromone as 13-amino acid peptide. The peptide sequence of the pheromone has been described through biochemical characterisation.",

keywords = "Quorum sensing, pheromone, bacillus, gene cloning, peptide purification, genome sequencing",

author = "Elham Esmaeilishirazifard and {De Vizio}, Daniela and Sterghios Moschos and Tajali Keshavarz",

year = "2017",

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T1 - Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis

AU - Esmaeilishirazifard, Elham

AU - De Vizio, Daniela

AU - Moschos, Sterghios

AU - Keshavarz, Tajali

PY - 2017/4/8

Y1 - 2017/4/8

N2 - Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS. The results obtained from bioinformatics studies on the whole genome sequence of this strain confirmed the presence of essential quorum sensing-related genes. Although polymorphism was verified in three proteins of this cluster, ComQ, precursor-ComX and ComP, the transcription factor ComA was confirmed as the most conserved protein. The cell–cell communication of B. licheniformis NCIMB-8874 was investigated through further elucidation of the ComX pheromone as 13-amino acid peptide. The peptide sequence of the pheromone has been described through biochemical characterisation.

AB - Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS. The results obtained from bioinformatics studies on the whole genome sequence of this strain confirmed the presence of essential quorum sensing-related genes. Although polymorphism was verified in three proteins of this cluster, ComQ, precursor-ComX and ComP, the transcription factor ComA was confirmed as the most conserved protein. The cell–cell communication of B. licheniformis NCIMB-8874 was investigated through further elucidation of the ComX pheromone as 13-amino acid peptide. The peptide sequence of the pheromone has been described through biochemical characterisation.

KW - Quorum sensing

KW - pheromone

KW - bacillus

KW - gene cloning

KW - peptide purification

KW - genome sequencing

U2 - 10.1186/s13568-017-0381-6

DO - 10.1186/s13568-017-0381-6

M3 - Article

SN - 2191-0855

VL - 7

JO - AMB Express

JF - AMB Express

IS - 78

ER -

Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis

Abstract

Keywords

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