Abstract
Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some special modifications, such as fucose-modification by fucosyltransferase (FucT). Three PtFucTs were annotated in the genome of P. tricornutum, PtFucT1 was located on the medial/trans-Golgi apparatus and PtFucT2-3 in the plastid stroma. Algal growth, biomass and photosynthesis efficiency were significantly inhibited in a knockout mutant of PtFucT1 (PtFucT1-KO). PtFucT1 played a role in non-core fucose modification of N-glycans. The knockout of PtFucT1 might affect the activity of PtGnTI in the complex and change the complex N-glycan to mannose type N-glycan. The study provided critical information for understanding the mechanism of protein N-glycosylation modification and using microalgae as an alternative ecofriendly cell factory to produce biopharmaceuticals.
Original language | English |
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Article number | 6 |
Number of pages | 18 |
Journal | Microbial Cell Factories |
Volume | 22 |
Issue number | 1 |
Early online date | 7 Jan 2023 |
DOIs | |
Publication status | Published - Dec 2023 |
Externally published | Yes |
Keywords
- Fucosyltransferase
- Glycoprotein
- Microbial cell factory
- N-glycan
- Phaeodactylum tricornutum