High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray

Laura Luque-Sastre, Kieran Jordan, Séamus Fanning, Edward M. Fox*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)

Abstract

High-throughput biochemical screening techniques are an important tool in phenotypic analysis of bacteria. New methods, simultaneously measuring many phenotype responses, increase the output of such investigations and allow a more complete overview of the bacterial phenotype, facilitating large-scale correlation to related genotypes. This chapter describes the application of OmniLog phenotype microarray analysis, a high-throughput assay for the phenotypic characterization of bacterial strains across a variety of different traits such as nutrient utilization and antimicrobial sensitivity, to Listeria species.

Original languageEnglish
Title of host publicationListeria Monocytogenes
Subtitle of host publicationMethods and Protocols
EditorsEdward M. Fox, Hélène Bierne, Beatrix Stessl
Place of PublicationNew York, NY
PublisherHumana Press Inc.
Pages107-113
Number of pages7
Edition2nd
ISBN (Electronic)9781071609828
ISBN (Print)9781071609811, 9781071609842
DOIs
Publication statusPublished - 26 Sept 2020

Publication series

NameMethods in Molecular Biology
PublisherHumana
Volume2220
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Antimicrobial sensitivity
  • Biochemical characterization
  • Nutrient utilization
  • OmniLog
  • Phenotype
  • Phenotypic microarray

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