High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format

Jessica A. Gray, P. Scott Chandry, John P. Bowman, Edward M. Fox*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Listeria monocytogenes is a foodborne pathogen capable of colonizing and persisting in the food production environment (FPE). While there are a variety of factors involved in L. monocytogenes’ ability to persist in FPE, the ability to form biofilms has the potential to increase their chance of survival and long-term colonization. Understanding the mechanisms involved in L. monocytogenes ability to form biofilms may potentially help food safety managers optimize control strategies targeting it in the FPE. In this chapter, a high-throughput method to determine L. monocytogenes ability to attach and form biofilms utilizing FPE-grade stainless steel is described. This method provides fast and efficient results, facilitating scaling up to large numbers of isolates to measure their ability to form biofilms, where lower-throughput approaches can then be utilized to further characterize isolates of interest.
Original languageEnglish
Title of host publicationListeria Monocytogenes
Subtitle of host publicationMethods and Protocols
EditorsEdward M. Fox, Hélène Bierne, Beatrix Stessl
Place of PublicationNew York, NY
PublisherHumana Press Inc.
Pages115-122
Number of pages8
Edition2nd
ISBN (Electronic)9781071609828
ISBN (Print)9781071609811, 9781071609842
DOIs
Publication statusPublished - 26 Sept 2020

Publication series

NameMethods in Molecular Biology
PublisherHumana
Volume2220
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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