Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens

Chong Li; Jikai Zhang; Haiyu Xie; Jingting Luo; Chen Fu; Ran Tao; Honglang Li; Yongqing (Richard) Fu

doi:10.3390/bios12070536

Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens

Chong Li, Jikai Zhang, Haiyu Xie, Jingting Luo, Chen Fu, Ran Tao, Honglang Li, Yongqing (Richard) Fu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.

Original language	English
Article number	536
Number of pages	11
Journal	Biosensors
Volume	12
Issue number	7
DOIs	https://doi.org/10.3390/bios12070536
Publication status	Published - 18 Jul 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

love wave
biosensors
CEA
AuNPs

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10.3390/bios12070536Licence: CC BY

Biosensors accepted manuscript.v7-0717 - clearAccepted author manuscript, 875 KBLicence: CC BY
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Cite this

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title = "Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens",

abstract = "A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.",

keywords = "love wave, biosensors, CEA, AuNPs",

author = "Chong Li and Jikai Zhang and Haiyu Xie and Jingting Luo and Chen Fu and Ran Tao and Honglang Li and Fu, \{Yongqing (Richard)\}",

note = "Funding information: This work was financially supported by the Research Project in Fundamental and Application Fields of Guangdong Province (Grant no. 2020A1515110561, 2019A1515111199), Research and Development Program of Guangdong Province (Grant no. 2020B0101040002), Key Research Program of Education Department for Guangdong Province (Grant no. 2020ZDZX2007), National Natural Science Foundation of China (NSFC Grant no. 12104320), Shenzhen Science \& Technology Project (Grant no. RCBS20200714114918249, GJHZ20200731095803010), Engineering and Physical Sciences Research Council of UK (EPSRC EP/P018998/1), Special Interest Group for Acoustofluidics from UK Fluids Network (EP/N032861/1), International Exchange Grant (IEC/NSFC/201078) through the Royal Society and NSFC, and EPSRC NetworkPlus in Digitalised Surface Manufacturing (EP/S036180/1).",

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doi = "10.3390/bios12070536",

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AU - Li, Chong

AU - Zhang, Jikai

AU - Xie, Haiyu

AU - Luo, Jingting

AU - Fu, Chen

AU - Tao, Ran

AU - Li, Honglang

AU - Fu, Yongqing (Richard)

N1 - Funding information: This work was financially supported by the Research Project in Fundamental and Application Fields of Guangdong Province (Grant no. 2020A1515110561, 2019A1515111199), Research and Development Program of Guangdong Province (Grant no. 2020B0101040002), Key Research Program of Education Department for Guangdong Province (Grant no. 2020ZDZX2007), National Natural Science Foundation of China (NSFC Grant no. 12104320), Shenzhen Science & Technology Project (Grant no. RCBS20200714114918249, GJHZ20200731095803010), Engineering and Physical Sciences Research Council of UK (EPSRC EP/P018998/1), Special Interest Group for Acoustofluidics from UK Fluids Network (EP/N032861/1), International Exchange Grant (IEC/NSFC/201078) through the Royal Society and NSFC, and EPSRC NetworkPlus in Digitalised Surface Manufacturing (EP/S036180/1).

PY - 2022/7/18

Y1 - 2022/7/18

N2 - A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.

AB - A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.

KW - love wave

KW - biosensors

KW - CEA

KW - AuNPs

UR - https://www.scopus.com/pages/publications/85135103931

U2 - 10.3390/bios12070536

DO - 10.3390/bios12070536

M3 - Article

SN - 2079-6374

VL - 12

JO - Biosensors

JF - Biosensors

IS - 7

M1 - 536

ER -

Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens

Abstract

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Keywords

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