Highly transparent liquid marble in liquid (HT-LMIL) as 3D miniaturized reactor for real-time bio-/chemical assays

Zhijian Zhao; Xiaoxue Yao; Wen Zhao; Bo Shi; Sreepathy Sridhar; Yuan Pu; Stevin Pramana; Dan Wang; Steven Wang

doi:10.1016/j.cej.2022.136417

Highly transparent liquid marble in liquid (HT-LMIL) as 3D miniaturized reactor for real-time bio-/chemical assays

Zhijian Zhao, Xiaoxue Yao, Wen Zhao, Bo Shi, Sreepathy Sridhar, Yuan Pu, Stevin Pramana, Dan Wang^*, Steven Wang

^*Corresponding author for this work

Mechanical and Construction Engineering Department

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

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Abstract

Traditional liquid marbles (LMs), are liquid droplets encapsulated by hydrophobic particles offering special three-dimensional (3D) stereo-architectures with structurally and functionally confined environments. However, the particle armors impede optical characterization in chemical analysis and process monitoring for fluid reactions. For the first time, we manipulated the liquid–solid interface by the paradigm of liquid marbles in liquid (LMIL) strategy, in which LMs are coated by diverse particles immersed in organic mediums rendering an optically transparent micro-reactor system without compromising on its original key features. We demonstrated comparable transmittance of highly transparent liquid marble in liquid (HT-LMIL) towards naked water droplets via UV–vis absorption spectroscopic detection. Besides, its function as an oil immersion lens gives higher resolution in optical spectroscopic characterization. The high optical transparency of LMIL enables real-time and in situ monitoring of yeast cells’ proliferation and viability via layer-by-layer, encouraging them to differentiate in suspension without anchoring. Such multifaceted characteristics of HT-LMIL based platform offer optically transparent and functionally promising micro/bio-reactors with future explorations for high-throughput chemical assays.

Original language	English
Article number	136417
Number of pages	8
Journal	Chemical Engineering Journal
Volume	443
Early online date	19 Apr 2022
DOIs	https://doi.org/10.1016/j.cej.2022.136417
Publication status	Published - 1 Sept 2022

Keywords

3D miniaturized reactor
Cell culture
Chemical assays
HT-LMIL
Liquid marble

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10.1016/j.cej.2022.136417

AAMAccepted author manuscript, 11.4 MBLicence: CC BY-NC-ND

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title = "Highly transparent liquid marble in liquid (HT-LMIL) as 3D miniaturized reactor for real-time bio-/chemical assays",

abstract = "Traditional liquid marbles (LMs), are liquid droplets encapsulated by hydrophobic particles offering special three-dimensional (3D) stereo-architectures with structurally and functionally confined environments. However, the particle armors impede optical characterization in chemical analysis and process monitoring for fluid reactions. For the first time, we manipulated the liquid–solid interface by the paradigm of liquid marbles in liquid (LMIL) strategy, in which LMs are coated by diverse particles immersed in organic mediums rendering an optically transparent micro-reactor system without compromising on its original key features. We demonstrated comparable transmittance of highly transparent liquid marble in liquid (HT-LMIL) towards naked water droplets via UV–vis absorption spectroscopic detection. Besides, its function as an oil immersion lens gives higher resolution in optical spectroscopic characterization. The high optical transparency of LMIL enables real-time and in situ monitoring of yeast cells{\textquoteright} proliferation and viability via layer-by-layer, encouraging them to differentiate in suspension without anchoring. Such multifaceted characteristics of HT-LMIL based platform offer optically transparent and functionally promising micro/bio-reactors with future explorations for high-throughput chemical assays.",

keywords = "3D miniaturized reactor, Cell culture, Chemical assays, HT-LMIL, Liquid marble",

author = "Zhijian Zhao and Xiaoxue Yao and Wen Zhao and Bo Shi and Sreepathy Sridhar and Yuan Pu and Stevin Pramana and Dan Wang and Steven Wang",

note = "Funding Information: Z.J. Zhao and X.X. Yao contributed equally to this work. We are grateful for final support from National Key Research and Development Program of China (2021YFC3001602). This research was also financially supported by grants 9610469 and 7005636 from the City University of Hong Kong.",

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T1 - Highly transparent liquid marble in liquid (HT-LMIL) as 3D miniaturized reactor for real-time bio-/chemical assays

AU - Zhao, Zhijian

AU - Yao, Xiaoxue

AU - Zhao, Wen

AU - Shi, Bo

AU - Sridhar, Sreepathy

AU - Pu, Yuan

AU - Pramana, Stevin

AU - Wang, Dan

AU - Wang, Steven

N1 - Funding Information: Z.J. Zhao and X.X. Yao contributed equally to this work. We are grateful for final support from National Key Research and Development Program of China (2021YFC3001602). This research was also financially supported by grants 9610469 and 7005636 from the City University of Hong Kong.

PY - 2022/9/1

Y1 - 2022/9/1

N2 - Traditional liquid marbles (LMs), are liquid droplets encapsulated by hydrophobic particles offering special three-dimensional (3D) stereo-architectures with structurally and functionally confined environments. However, the particle armors impede optical characterization in chemical analysis and process monitoring for fluid reactions. For the first time, we manipulated the liquid–solid interface by the paradigm of liquid marbles in liquid (LMIL) strategy, in which LMs are coated by diverse particles immersed in organic mediums rendering an optically transparent micro-reactor system without compromising on its original key features. We demonstrated comparable transmittance of highly transparent liquid marble in liquid (HT-LMIL) towards naked water droplets via UV–vis absorption spectroscopic detection. Besides, its function as an oil immersion lens gives higher resolution in optical spectroscopic characterization. The high optical transparency of LMIL enables real-time and in situ monitoring of yeast cells’ proliferation and viability via layer-by-layer, encouraging them to differentiate in suspension without anchoring. Such multifaceted characteristics of HT-LMIL based platform offer optically transparent and functionally promising micro/bio-reactors with future explorations for high-throughput chemical assays.

AB - Traditional liquid marbles (LMs), are liquid droplets encapsulated by hydrophobic particles offering special three-dimensional (3D) stereo-architectures with structurally and functionally confined environments. However, the particle armors impede optical characterization in chemical analysis and process monitoring for fluid reactions. For the first time, we manipulated the liquid–solid interface by the paradigm of liquid marbles in liquid (LMIL) strategy, in which LMs are coated by diverse particles immersed in organic mediums rendering an optically transparent micro-reactor system without compromising on its original key features. We demonstrated comparable transmittance of highly transparent liquid marble in liquid (HT-LMIL) towards naked water droplets via UV–vis absorption spectroscopic detection. Besides, its function as an oil immersion lens gives higher resolution in optical spectroscopic characterization. The high optical transparency of LMIL enables real-time and in situ monitoring of yeast cells’ proliferation and viability via layer-by-layer, encouraging them to differentiate in suspension without anchoring. Such multifaceted characteristics of HT-LMIL based platform offer optically transparent and functionally promising micro/bio-reactors with future explorations for high-throughput chemical assays.

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Highly transparent liquid marble in liquid (HT-LMIL) as 3D miniaturized reactor for real-time bio-/chemical assays

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