TY - JOUR
T1 - Human Topoisomerase IIα and IIβ Interact with the C-Terminal Region of p53
AU - Cowell, Ian G.
AU - Okorokov, Andrei L.
AU - Cutts, Sarah A.
AU - Padget, Kay
AU - Bell, Margaret
AU - Milner, Jo
AU - Austin, Caroline A.
PY - 2000/2/25
Y1 - 2000/2/25
N2 - The p53 tumor suppressor protein is a critical regulator of cell cycle progression and apoptosis following exposure of cells to DNA damaging agents such as ionizing radiation or anticancer drugs. An important group of anticancer drugs, including compounds such as etoposide and doxorubicin (Adriamycin), interacts with DNA topoisomerase II (topo II), causing the accumulation of enzyme-DNA adducts that ultimately lead to double-strand breaks and cell death via apoptosis. Human topo IIβ has previously been shown to interact with p53, and we have extended this analysis to show that both topo IIα and IIβ interact with p53 in vivo and in vitro. Furthermore, we show that the regulatory C-terminal basic region of p53 (residues 364–393) is necessary and sufficient for interaction with DNA topo II.
AB - The p53 tumor suppressor protein is a critical regulator of cell cycle progression and apoptosis following exposure of cells to DNA damaging agents such as ionizing radiation or anticancer drugs. An important group of anticancer drugs, including compounds such as etoposide and doxorubicin (Adriamycin), interacts with DNA topoisomerase II (topo II), causing the accumulation of enzyme-DNA adducts that ultimately lead to double-strand breaks and cell death via apoptosis. Human topo IIβ has previously been shown to interact with p53, and we have extended this analysis to show that both topo IIα and IIβ interact with p53 in vivo and in vitro. Furthermore, we show that the regulatory C-terminal basic region of p53 (residues 364–393) is necessary and sufficient for interaction with DNA topo II.
UR - https://www.scopus.com/pages/publications/0034711877
U2 - 10.1006/excr.1999.4772
DO - 10.1006/excr.1999.4772
M3 - Article
SN - 0014-4827
VL - 255
SP - 86
EP - 94
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -
