Hyperthermia Suppresses Post - In Vitro Proliferation and Tumor Growth in Murine Malignant Melanoma and Colon Carcinoma

Theodora Mantso; Stavros Vasileiadis; Evangeli Lampri; Sotiris Botaitis; Sebachedin Perente; Constantinos Simopoulos; Katerina Chlichlia; Aglaia Pappa; Mihalis Panagiotidis

doi:10.21873/anticanres.13347

Hyperthermia Suppresses Post - In Vitro Proliferation and Tumor Growth in Murine Malignant Melanoma and Colon Carcinoma

Theodora Mantso, Stavros Vasileiadis, Evangeli Lampri, Sotiris Botaitis, Sebachedin Perente, Constantinos Simopoulos, Katerina Chlichlia, Aglaia Pappa, Mihalis Panagiotidis

Applied Sciences Department

Research output: Contribution to journal › Article › peer-review

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Abstract

Background: Several studies have highlighted hyperthermia’s ability to enhance the effectiveness of radiation and chemotherapy in various in vitro and in vivo cancer models.

Materials and Methods: In vivo murine models of malignant melanoma and colon carcinoma were utilized for demonstrating hyperthermia’s therapeutic effectiveness by examining levels of caspase 3, COX-2 and phospho-H2A.X (Ser139) as endpoints of apoptosis, proliferation and DNA damage respectively.

Results: Hyperthermia induced in vitro cytotoxicity in malignant melanoma (B16-F10) and colon carcinoma (CT26) cell lines. In addition, it reduced post-in vitro proliferation and suppression of tumor growth by inducing the expression of caspase-3 and phospho-H2A.X (Ser139) while reducing the expression of COX-2 in both murine cancer models.

Conclusion: Hyperthermia can exert therapeutic effectiveness against melanoma and colon carcinoma by inhibiting a number of critical cellular cascades including apoptosis, proliferation and DNA damage.

Original language	English
Pages (from-to)	2307-2315
Number of pages	9
Journal	Anticancer Research
Volume	39
Issue number	5
DOIs	https://doi.org/10.21873/anticanres.13347
Publication status	Published - May 2019

Keywords

Hyperthermia
malignant melanoma
colon carcinoma
experimental cancer therapeutics
proliferation
apoptosis
DNA damage

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Hyperthermia Suppresses Post - In Vitro Proliferation and Tumor Growth in Murine Malignant Melanoma and Colon Carcinoma",

abstract = "Background: Several studies have highlighted hyperthermia{\textquoteright}s ability to enhance the effectiveness of radiation and chemotherapy in various in vitro and in vivo cancer models.Materials and Methods: In vivo murine models of malignant melanoma and colon carcinoma were utilized for demonstrating hyperthermia{\textquoteright}s therapeutic effectiveness by examining levels of caspase 3, COX-2 and phospho-H2A.X (Ser139) as endpoints of apoptosis, proliferation and DNA damage respectively.Results: Hyperthermia induced in vitro cytotoxicity in malignant melanoma (B16-F10) and colon carcinoma (CT26) cell lines. In addition, it reduced post-in vitro proliferation and suppression of tumor growth by inducing the expression of caspase-3 and phospho-H2A.X (Ser139) while reducing the expression of COX-2 in both murine cancer models.Conclusion: Hyperthermia can exert therapeutic effectiveness against melanoma and colon carcinoma by inhibiting a number of critical cellular cascades including apoptosis, proliferation and DNA damage.",

keywords = "Hyperthermia, malignant melanoma, colon carcinoma, experimental cancer therapeutics, proliferation, apoptosis, DNA damage",

author = "Theodora Mantso and Stavros Vasileiadis and Evangeli Lampri and Sotiris Botaitis and Sebachedin Perente and Constantinos Simopoulos and Katerina Chlichlia and Aglaia Pappa and Mihalis Panagiotidis",

year = "2019",

month = may,

doi = "10.21873/anticanres.13347",

language = "English",

volume = "39",

pages = "2307--2315",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

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TY - JOUR

T1 - Hyperthermia Suppresses Post - In Vitro Proliferation and Tumor Growth in Murine Malignant Melanoma and Colon Carcinoma

AU - Mantso, Theodora

AU - Vasileiadis, Stavros

AU - Lampri, Evangeli

AU - Botaitis, Sotiris

AU - Perente, Sebachedin

AU - Simopoulos, Constantinos

AU - Chlichlia, Katerina

AU - Pappa, Aglaia

AU - Panagiotidis, Mihalis

PY - 2019/5

Y1 - 2019/5

N2 - Background: Several studies have highlighted hyperthermia’s ability to enhance the effectiveness of radiation and chemotherapy in various in vitro and in vivo cancer models.Materials and Methods: In vivo murine models of malignant melanoma and colon carcinoma were utilized for demonstrating hyperthermia’s therapeutic effectiveness by examining levels of caspase 3, COX-2 and phospho-H2A.X (Ser139) as endpoints of apoptosis, proliferation and DNA damage respectively.Results: Hyperthermia induced in vitro cytotoxicity in malignant melanoma (B16-F10) and colon carcinoma (CT26) cell lines. In addition, it reduced post-in vitro proliferation and suppression of tumor growth by inducing the expression of caspase-3 and phospho-H2A.X (Ser139) while reducing the expression of COX-2 in both murine cancer models.Conclusion: Hyperthermia can exert therapeutic effectiveness against melanoma and colon carcinoma by inhibiting a number of critical cellular cascades including apoptosis, proliferation and DNA damage.

AB - Background: Several studies have highlighted hyperthermia’s ability to enhance the effectiveness of radiation and chemotherapy in various in vitro and in vivo cancer models.Materials and Methods: In vivo murine models of malignant melanoma and colon carcinoma were utilized for demonstrating hyperthermia’s therapeutic effectiveness by examining levels of caspase 3, COX-2 and phospho-H2A.X (Ser139) as endpoints of apoptosis, proliferation and DNA damage respectively.Results: Hyperthermia induced in vitro cytotoxicity in malignant melanoma (B16-F10) and colon carcinoma (CT26) cell lines. In addition, it reduced post-in vitro proliferation and suppression of tumor growth by inducing the expression of caspase-3 and phospho-H2A.X (Ser139) while reducing the expression of COX-2 in both murine cancer models.Conclusion: Hyperthermia can exert therapeutic effectiveness against melanoma and colon carcinoma by inhibiting a number of critical cellular cascades including apoptosis, proliferation and DNA damage.

KW - Hyperthermia

KW - malignant melanoma

KW - colon carcinoma

KW - experimental cancer therapeutics

KW - proliferation

KW - apoptosis

KW - DNA damage

U2 - 10.21873/anticanres.13347

DO - 10.21873/anticanres.13347

M3 - Article

SN - 0250-7005

VL - 39

SP - 2307

EP - 2315

JO - Anticancer Research

JF - Anticancer Research

IS - 5

ER -

Hyperthermia Suppresses Post - In Vitro Proliferation and Tumor Growth in Murine Malignant Melanoma and Colon Carcinoma

Abstract

Keywords

UN SDGs

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