TY - JOUR
T1 - Corynebacterium guaraldiae sp. nov.
T2 - a new species of Corynebacterium from human infections
AU - de Oliveira Sant'Anna, Lincoln
AU - Dos Santos, Louisy Sanches
AU - Araújo, Max Roberto Batista
AU - da Rocha, Danilo Jobim Passos Gil
AU - Ramos, Juliana Nunes
AU - Baio, Paulo Victor Pereira
AU - Del Peloso, Pedro Fernandez
AU - da Costa Ferreira Leite, Cassiana
AU - Peixoto, Renata Stavrakakis
AU - Almuzara, Marisa
AU - Vay, Carlos
AU - Barberis, Claudia
AU - Sangal, Vartul
AU - Burkovski, Andreas
AU - Aguiar, Eric Roberto Guimarães Rocha
AU - Mattos-Guaraldi, Ana Luíza
AU - Pacheco, Luis Gustavo Carvalho
AU - Vieira, Verônica Viana
N1 - Funding information: This work was supported by Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES) # 88882.450376/2019–01, and # 88882.315426/2019–01, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) # E-26/202.087/2020, # E 26/210.889/2019, and # E-26/211.629/2015, Fundação Oswaldo Cruz (INOVA/FIOCRUZ) # 7481297331, Conselho Nacional de Desenvolvimento Científco e Tecnológico (CNPq) # 309948/2018–5, and Secretaria de Ciencia y Técnica de la Universidad de Buenos Aires (UBACyT) # 20020170100109 BA.
PY - 2023/6/1
Y1 - 2023/6/1
N2 - Non-diphtheria Corynebacterium species (NDC) belonging to the human skin and mucosa microbiota are frequently neglected as contaminants. However, reports of human infections by Corynebacterium spp. have increased considerably in recent years. In this study, a group of six NDC isolates of urine (n = 5) and sebaceous cyst (n = 1) from two South American countries were identified at genus level or misidentified based on API® Coryne and genetic/molecular analyses. The 16S rRNA (99.09-99.56%) and rpoB (96.18-97.14%) gene sequence similarities of the isolates were higher when compared with Corynebacterium aurimucosum DSM 44532 T. Multilocus sequence analysis (MLSA) indicated that these six NDC isolates compose a distinctive phylogenetic clade. Genome-based taxonomic analysis with the whole-genome sequences was able to separate these six isolates from other known Corynebacterium type strains. Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between closely related type strains and the six isolates were considerably lower than the currently recommended threshold values for species circumscription. Phylogenetic and genomic taxonomy analyses indicated these microorganisms as a novel Corynebacterium species, for which we formally propose the name Corynebacterium guaraldiae sp. nov. with isolate 13 T (= CBAS 827 T = CCBH 35012 T) as type strain.
AB - Non-diphtheria Corynebacterium species (NDC) belonging to the human skin and mucosa microbiota are frequently neglected as contaminants. However, reports of human infections by Corynebacterium spp. have increased considerably in recent years. In this study, a group of six NDC isolates of urine (n = 5) and sebaceous cyst (n = 1) from two South American countries were identified at genus level or misidentified based on API® Coryne and genetic/molecular analyses. The 16S rRNA (99.09-99.56%) and rpoB (96.18-97.14%) gene sequence similarities of the isolates were higher when compared with Corynebacterium aurimucosum DSM 44532 T. Multilocus sequence analysis (MLSA) indicated that these six NDC isolates compose a distinctive phylogenetic clade. Genome-based taxonomic analysis with the whole-genome sequences was able to separate these six isolates from other known Corynebacterium type strains. Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between closely related type strains and the six isolates were considerably lower than the currently recommended threshold values for species circumscription. Phylogenetic and genomic taxonomy analyses indicated these microorganisms as a novel Corynebacterium species, for which we formally propose the name Corynebacterium guaraldiae sp. nov. with isolate 13 T (= CBAS 827 T = CCBH 35012 T) as type strain.
KW - Classification
KW - Corynebacterium
KW - Corynebacterium guaraldiae sp. nov
KW - Epidermal cyst
KW - Urinary tract infection
UR - http://www.scopus.com/inward/record.url?scp=85149222202&partnerID=8YFLogxK
U2 - 10.1007/s42770-023-00938-y
DO - 10.1007/s42770-023-00938-y
M3 - Article
C2 - 36869213
SN - 1517-8382
VL - 54
SP - 779
EP - 790
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
IS - 2
ER -