Corynebacterium guaraldiae sp. nov. a new species of Corynebacterium from human infections

Lincoln de Oliveira Sant'Anna*, Louisy Sanches Dos Santos, Max Roberto Batista Araújo, Danilo Jobim Passos Gil da Rocha, Juliana Nunes Ramos, Paulo Victor Pereira Baio, Pedro Fernandez Del Peloso, Cassiana da Costa Ferreira Leite, Renata Stavrakakis Peixoto, Marisa Almuzara, Carlos Vay, Claudia Barberis, Vartul Sangal, Andreas Burkovski, Eric Roberto Guimarães Rocha Aguiar, Ana Luíza Mattos-Guaraldi, Luis Gustavo Carvalho Pacheco, Verônica Viana Vieira

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Non-diphtheria Corynebacterium species (NDC) belonging to the human skin and mucosa microbiota are frequently neglected as contaminants. However, reports of human infections by Corynebacterium spp. have increased considerably in recent years. In this study, a group of six NDC isolates of urine (n = 5) and sebaceous cyst (n = 1) from two South American countries were identified at genus level or misidentified based on API® Coryne and genetic/molecular analyses. The 16S rRNA (99.09-99.56%) and rpoB (96.18-97.14%) gene sequence similarities of the isolates were higher when compared with Corynebacterium aurimucosum DSM 44532 T. Multilocus sequence analysis (MLSA) indicated that these six NDC isolates compose a distinctive phylogenetic clade. Genome-based taxonomic analysis with the whole-genome sequences was able to separate these six isolates from other known Corynebacterium type strains. Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between closely related type strains and the six isolates were considerably lower than the currently recommended threshold values for species circumscription. Phylogenetic and genomic taxonomy analyses indicated these microorganisms as a novel Corynebacterium species, for which we formally propose the name Corynebacterium guaraldiae sp. nov. with isolate 13 T (= CBAS 827 T  = CCBH 35012 T) as type strain.

Original languageEnglish
Pages (from-to)779-790
Number of pages12
JournalBrazilian Journal of Microbiology
Volume54
Issue number2
Early online date4 Mar 2023
DOIs
Publication statusPublished - 1 Jun 2023

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