Identification of B2 Bradykinin Binding Sites on Cultured Cortical Astrocytes

A. J. Cholewinski; G. Stevens; Alison McDermott; G. P. Wilkin

doi:10.1111/j.1471-4159.1991.tb08314.x

Identification of B2 Bradykinin Binding Sites on Cultured Cortical Astrocytes

A. J. Cholewinski, G. Stevens, Alison McDermott, G. P. Wilkin

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 ± 2.6 nM and a Bmax of 352.2 ±30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.

Original language	English
Pages (from-to)	1456-1458
Journal	Journal of Neurochemistry
Volume	57
Issue number	4
DOIs	https://doi.org/10.1111/j.1471-4159.1991.tb08314.x
Publication status	Published - Oct 1991

Keywords

Bradykinin
Bradykinin receptors
Astrocytes
Cell culture

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10.1111/j.1471-4159.1991.tb08314.x

https://doi.org/10.1111/j.1471-4159.1991.tb08314.x

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abstract = "Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 ± 2.6 nM and a Bmax of 352.2 ±30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.",

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AU - Cholewinski, A. J.

AU - Stevens, G.

AU - McDermott, Alison

AU - Wilkin, G. P.

PY - 1991/10

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N2 - Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 ± 2.6 nM and a Bmax of 352.2 ±30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.

AB - Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 ± 2.6 nM and a Bmax of 352.2 ±30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.

KW - Bradykinin

KW - Bradykinin receptors

KW - Astrocytes

KW - Cell culture

UR - https://www.scopus.com/pages/publications/0025925101

U2 - 10.1111/j.1471-4159.1991.tb08314.x

DO - 10.1111/j.1471-4159.1991.tb08314.x

M3 - Article

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EP - 1458

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

IS - 4

ER -

Identification of B2 Bradykinin Binding Sites on Cultured Cortical Astrocytes

Abstract

Keywords

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