A new approach to measurement of Eu and Sm monoclonal antibody (MAb) labels by Flow Injection ICP-MS following dual immunoassay for the prostate cancer markers, free prostate specific antigen (fPSA) and total prostate specific antigen (tPSA), based on a sandwich-format immunoassay is described. Limits of detection were 0.01 μg/L (0.22 ng/L Eu) for fPSA and 0.02 μg/L (0.11 ng/L Sm) for tPSA, and precision ranged from 0.75% to 3.68% for fPSA (Eu) and from 1.04% to 5.47% for tPSA (Sm). In general, the approach was competitive with the standard time-resolved fluorimetric (TRF) method as applied in clinical chemistry (1). Accuracy was assessed through comparative studies (TRF) and analysis of reference sera (UK NEQAS).
|Number of pages||7|
|Journal||Canadian Journal of Analytical Sciences and Spectroscopy|
|Publication status||Published - Jan 2004|