Immunoassay with FI-ICP-MS detection - Measurement of free and total prostate specific antigen in human serum

Robert W. Hutchinson; Renli Ma; Cameron W. McLeod; Anthony Milford-Ward; David Lee

Immunoassay with FI-ICP-MS detection - Measurement of free and total prostate specific antigen in human serum

Robert W. Hutchinson, Renli Ma, Cameron W. McLeod^*, Anthony Milford-Ward, David Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A new approach to measurement of Eu and Sm monoclonal antibody (MAb) labels by Flow Injection ICP-MS following dual immunoassay for the prostate cancer markers, free prostate specific antigen (fPSA) and total prostate specific antigen (tPSA), based on a sandwich-format immunoassay is described. Limits of detection were 0.01 μg/L (0.22 ng/L Eu) for fPSA and 0.02 μg/L (0.11 ng/L Sm) for tPSA, and precision ranged from 0.75% to 3.68% for fPSA (Eu) and from 1.04% to 5.47% for tPSA (Sm). In general, the approach was competitive with the standard time-resolved fluorimetric (TRF) method as applied in clinical chemistry (1). Accuracy was assessed through comparative studies (TRF) and analysis of reference sera (UK NEQAS).

Original language	English
Pages (from-to)	429-435
Number of pages	7
Journal	Canadian Journal of Analytical Sciences and Spectroscopy
Volume	49
Issue number	6
Publication status	Published - Jan 2004
Externally published	Yes

Keywords

Antibodies
Flow injection
Human serum
Inductively coupled plasma mass spectrometry
Prostate cancer

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{f39213809216408e96e605fc6e5d9116,

title = "Immunoassay with FI-ICP-MS detection - Measurement of free and total prostate specific antigen in human serum",

abstract = "A new approach to measurement of Eu and Sm monoclonal antibody (MAb) labels by Flow Injection ICP-MS following dual immunoassay for the prostate cancer markers, free prostate specific antigen (fPSA) and total prostate specific antigen (tPSA), based on a sandwich-format immunoassay is described. Limits of detection were 0.01 μg/L (0.22 ng/L Eu) for fPSA and 0.02 μg/L (0.11 ng/L Sm) for tPSA, and precision ranged from 0.75% to 3.68% for fPSA (Eu) and from 1.04% to 5.47% for tPSA (Sm). In general, the approach was competitive with the standard time-resolved fluorimetric (TRF) method as applied in clinical chemistry (1). Accuracy was assessed through comparative studies (TRF) and analysis of reference sera (UK NEQAS).",

keywords = "Antibodies, Flow injection, Human serum, Inductively coupled plasma mass spectrometry, Prostate cancer",

author = "Hutchinson, {Robert W.} and Renli Ma and McLeod, {Cameron W.} and Anthony Milford-Ward and David Lee",

year = "2004",

month = jan,

language = "English",

volume = "49",

pages = "429--435",

journal = "Canadian Journal of Analytical Sciences and Spectroscopy",

issn = "1205-6685",

publisher = "Spectroscopy Society of Canada",

number = "6",

}

TY - JOUR

T1 - Immunoassay with FI-ICP-MS detection - Measurement of free and total prostate specific antigen in human serum

AU - Hutchinson, Robert W.

AU - Ma, Renli

AU - McLeod, Cameron W.

AU - Milford-Ward, Anthony

AU - Lee, David

PY - 2004/1

Y1 - 2004/1

N2 - A new approach to measurement of Eu and Sm monoclonal antibody (MAb) labels by Flow Injection ICP-MS following dual immunoassay for the prostate cancer markers, free prostate specific antigen (fPSA) and total prostate specific antigen (tPSA), based on a sandwich-format immunoassay is described. Limits of detection were 0.01 μg/L (0.22 ng/L Eu) for fPSA and 0.02 μg/L (0.11 ng/L Sm) for tPSA, and precision ranged from 0.75% to 3.68% for fPSA (Eu) and from 1.04% to 5.47% for tPSA (Sm). In general, the approach was competitive with the standard time-resolved fluorimetric (TRF) method as applied in clinical chemistry (1). Accuracy was assessed through comparative studies (TRF) and analysis of reference sera (UK NEQAS).

AB - A new approach to measurement of Eu and Sm monoclonal antibody (MAb) labels by Flow Injection ICP-MS following dual immunoassay for the prostate cancer markers, free prostate specific antigen (fPSA) and total prostate specific antigen (tPSA), based on a sandwich-format immunoassay is described. Limits of detection were 0.01 μg/L (0.22 ng/L Eu) for fPSA and 0.02 μg/L (0.11 ng/L Sm) for tPSA, and precision ranged from 0.75% to 3.68% for fPSA (Eu) and from 1.04% to 5.47% for tPSA (Sm). In general, the approach was competitive with the standard time-resolved fluorimetric (TRF) method as applied in clinical chemistry (1). Accuracy was assessed through comparative studies (TRF) and analysis of reference sera (UK NEQAS).

KW - Antibodies

KW - Flow injection

KW - Human serum

KW - Inductively coupled plasma mass spectrometry

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=27144497500&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:27144497500

SN - 1205-6685

VL - 49

SP - 429

EP - 435

JO - Canadian Journal of Analytical Sciences and Spectroscopy

JF - Canadian Journal of Analytical Sciences and Spectroscopy

IS - 6

ER -

Immunoassay with FI-ICP-MS detection - Measurement of free and total prostate specific antigen in human serum

Abstract

Keywords

UN SDGs

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