Immunoassay with FI-ICP-MS detection - Measurement of free and total prostate specific antigen in human serum

Robert W. Hutchinson, Renli Ma, Cameron W. McLeod*, Anthony Milford-Ward, David Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

A new approach to measurement of Eu and Sm monoclonal antibody (MAb) labels by Flow Injection ICP-MS following dual immunoassay for the prostate cancer markers, free prostate specific antigen (fPSA) and total prostate specific antigen (tPSA), based on a sandwich-format immunoassay is described. Limits of detection were 0.01 μg/L (0.22 ng/L Eu) for fPSA and 0.02 μg/L (0.11 ng/L Sm) for tPSA, and precision ranged from 0.75% to 3.68% for fPSA (Eu) and from 1.04% to 5.47% for tPSA (Sm). In general, the approach was competitive with the standard time-resolved fluorimetric (TRF) method as applied in clinical chemistry (1). Accuracy was assessed through comparative studies (TRF) and analysis of reference sera (UK NEQAS).

Original languageEnglish
Pages (from-to)429-435
Number of pages7
JournalCanadian Journal of Analytical Sciences and Spectroscopy
Volume49
Issue number6
Publication statusPublished - Jan 2004
Externally publishedYes

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