TY - JOUR
T1 - Immunochemical and Mass Spectrometric Analysis of Nε-(Carboxymethyl)lysine Content of AGE−BSA Systems Prepared with and without Selected Antiglycation Agents
AU - Srey, Chou
AU - Haughey, Simon
AU - Connolly, Lisa
AU - del Castillo, Maria Dolores
AU - Elliott, Christopher
AU - Ames, Jenny
PY - 2010
Y1 - 2010
N2 - The present study was designed to compare surface plasmon resonance (SPR) biosensor, enzyme-linked immunosorbent assay (ELISA), and ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods for the analysis of N(epsilon)-(carboxymethyplysine (CML) in glucose bovine serum albumin (BSA) model systems and to investigate the possible inhibitory effect of selected compounds (a-tocopherol, ferulic acid, rutin, thiamin, thiamin monophosphate, and thiamin pyrophosphate) on CML formation. The reported levels of CML detected were dependent upon the method of analysis employed. The highest reported concentrations were obtained with the SPA biosensor, whereas the lowest were found by ELISA. However, a high correlation was observed between these two immunochemical procedures. CML concentrations were dependent upon the type and concentration of the candidate CML inhibitor. All inhibitory compounds investigated, with the exception of a-tocopherol, decreased the level of CML formation in the glucose BSA system.
AB - The present study was designed to compare surface plasmon resonance (SPR) biosensor, enzyme-linked immunosorbent assay (ELISA), and ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods for the analysis of N(epsilon)-(carboxymethyplysine (CML) in glucose bovine serum albumin (BSA) model systems and to investigate the possible inhibitory effect of selected compounds (a-tocopherol, ferulic acid, rutin, thiamin, thiamin monophosphate, and thiamin pyrophosphate) on CML formation. The reported levels of CML detected were dependent upon the method of analysis employed. The highest reported concentrations were obtained with the SPA biosensor, whereas the lowest were found by ELISA. However, a high correlation was observed between these two immunochemical procedures. CML concentrations were dependent upon the type and concentration of the candidate CML inhibitor. All inhibitory compounds investigated, with the exception of a-tocopherol, decreased the level of CML formation in the glucose BSA system.
KW - N(epsilon)-(Carboxymethyl)lysine (CML)
KW - advanced glycation endproducts (AGEs)
KW - CML inhibitor
KW - enzyme-linked immunosorbent assay (ELISA)
KW - surface plasmon resonance (SPR) biosensor
KW - ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
U2 - 10.1021/jf102543g
DO - 10.1021/jf102543g
M3 - Article
SN - 0021-8561
VL - 58
SP - 11955
EP - 11961
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 22
ER -