TY - JOUR
T1 - Inhibitory activities of three Malaysian edible seaweeds on lipase and α-amylase
AU - Balasubramaniam, Vimala
AU - Mustar, Suraiami
AU - Mustafa Khalid, Norhayati
AU - Abd Rashed, Aswir
AU - Mohd Noh, Mohd Fairulnizal
AU - Wilcox, Matthew D.
AU - Chater, Peter I.
AU - Brownlee, Iain A.
AU - Pearson, Jeffrey P.
PY - 2013/10/1
Y1 - 2013/10/1
N2 - Ethanol extracts, dried powders and fibres (total and soluble fibre) of the tropical red algae Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum were analysed for their effect on lipase and α-amylase activity using turbidimetric method and dinitrosalicylic acid (DNS) assay, respectively. The nutrient composition analyses were determined using standard methods. The ethanol extract of dried K. striatus (Ks-III) showed the highest reduction in lipase activity with 92 % inhibition followed by seaweed powders (K. alvarezii (Ka-III), K. striatus (Ks-III) and E. denticulatum (Ed-III)) with average inhibition of 60 %. Soluble fibres of K. alvarezii (Ka-V) and E. denticulatum (Ed-V) showed significant inhibition with 60 and 57 % reduction, respectively. Only the ethanol extract of fresh E. denticulatum (Ed-I) showed 88 % inhibition of α-amylase. Nutritional component analyses showed that all three seaweeds are low in crude fat, suggesting the possible use of seaweed as a dietary supplement and for potential weight and glycaemia management.
AB - Ethanol extracts, dried powders and fibres (total and soluble fibre) of the tropical red algae Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum were analysed for their effect on lipase and α-amylase activity using turbidimetric method and dinitrosalicylic acid (DNS) assay, respectively. The nutrient composition analyses were determined using standard methods. The ethanol extract of dried K. striatus (Ks-III) showed the highest reduction in lipase activity with 92 % inhibition followed by seaweed powders (K. alvarezii (Ka-III), K. striatus (Ks-III) and E. denticulatum (Ed-III)) with average inhibition of 60 %. Soluble fibres of K. alvarezii (Ka-V) and E. denticulatum (Ed-V) showed significant inhibition with 60 and 57 % reduction, respectively. Only the ethanol extract of fresh E. denticulatum (Ed-I) showed 88 % inhibition of α-amylase. Nutritional component analyses showed that all three seaweeds are low in crude fat, suggesting the possible use of seaweed as a dietary supplement and for potential weight and glycaemia management.
KW - α-Amylase
KW - Dinitrosalicylic acid assay
KW - Lipase
KW - Nutrient composition
KW - Seaweed
KW - Turbidimetric method
UR - http://www.scopus.com/inward/record.url?scp=84883654526&partnerID=8YFLogxK
U2 - 10.1007/s10811-012-9964-4
DO - 10.1007/s10811-012-9964-4
M3 - Article
AN - SCOPUS:84883654526
SN - 0921-8971
VL - 25
SP - 1405
EP - 1412
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
IS - 5
ER -