Inhibitory activity of extracts of Hebridean brown seaweeds on lipase activity

Peter Ian Chater*, Mathew Wilcox, Paul Cherry, Andrew Herford, Suraiami Mustar, Hannah Wheater, Iain Brownlee, Chris Seal, Jeffrey Pearson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)
430 Downloads (Pure)

Abstract

The effect of three Hebridean brown seaweeds on lipase activity was assessed using a turbidimetric lipase activity assay and an in vitro simulation of the upper digestive tract. The preparations of Ascophyllum nodosum, Fucus vesiculosus, and Pelvetia canaliculata were tested; whole seaweed homogenate, sodium carbonate extract, and ethanol extracts (pellet and supernatant were tested separately). All extracts showed significant inhibition of lipase, suggesting multiple bioactive agents, potentially including alginates, fucoidans, and polyphenols. Whole homogenate extract of F. vesiculosus was the most potent inhibitor of Lipase (IC50 = 0.119 mg mL-1), followed by ethanol supernatant (IC50 = 0.159 mg mL-1) while ethanol pellet and sodium carbonate extract showed relatively weaker inhibition (IC50 = 0.360 mg mL-1 and IC50 = 0.969 mg mL-1 respectively). For A. nodosum and P. canaliculata, strongest inhibition occurred with ethanol pellet (IC50 = 0.238 and 0.228 mg mL−1, respectively). These inhibitory effects were validated in a model gut system. The data presented herein suggests the use of seaweed as a potential weight management tool is deserving of further investigation.

Original languageEnglish
Pages (from-to)1303-1313
Number of pages11
JournalJournal of Applied Phycology
Volume28
Issue number2
Early online date26 May 2015
DOIs
Publication statusPublished - 1 Apr 2016
Externally publishedYes

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