TY - JOUR
T1 - Integrated label-free erbium-doped fiber laser biosensing system for detection of single cell Staphylococcus aureus
AU - Liu, Jiandong
AU - Liu, Bin
AU - Liu, Juan
AU - He, Xing-Dao
AU - Yuan, Jinhui
AU - Ghassemlooy, Zabih
AU - Torun, Hamdi
AU - Fu, Yong-Qing
AU - Dai, Xuewu
AU - Ng, Wai Pang
AU - Binns, Richard
AU - Wu, Qiang
N1 - Funding information: This work was supported by the National Natural Science Foundation of China (NSFC) (11864025, 62175097, 62065013 and 62163029); Natural Science Foundation of Jiangxi Province (20212BAB202024 and 20192ACB20031); Royal Society International Exchanges 2020 Cost Share (NSFC) (IEC\NSFC\201015).
PY - 2023/5/15
Y1 - 2023/5/15
N2 - A critical challenge to realize ultra-high sensitivity with optical fiber interferometers for label free biosensing is to achieve high quality factors (Q-factor) in liquid. In this work a high Q-factor of 105, which significantly improves the detection resolution is described based on a structure of single mode -core-only -single mode fiber (SCS) with its multimode (or Mach-Zehnder) interference effect as a filter that is integrated into an erbium-doped fiber laser (EDFL) system for excitation. In the case study, the section of core-only fiber is functionalized with porcine immunoglobulin G (IgG) antibodies, which could selectively bind to bacterial pathogen of Staphylococcus aureus (S. aureus). The developed microfiber-based biosensing platform called SCS-based EDFL biosensors can effectively detect concentrations of S. aureus from 10 to 105 CFU/mL, with a responsivity of 42.6 p.m./(CFU/mL) for the wavelength shift of the measured spectrum. The limit of detection (LoD) is estimated as 7.3 CFU/mL based on the measurement of S. aureus with minimum concentration of 10 CFU/mL. In addition, when a lower concentration of 1 CFU/mL is applied to the biosensor, a wavelength shift of 0.12 nm is observed in 10% of samples (1/10), indicating actual LoD of 1 CFU/mL for the proposed biosensor. Attributed to its good sensitivity, stability, reproducibility and specificity, the proposed EDFL based biosensing platform has great potentials for diagnostics.
AB - A critical challenge to realize ultra-high sensitivity with optical fiber interferometers for label free biosensing is to achieve high quality factors (Q-factor) in liquid. In this work a high Q-factor of 105, which significantly improves the detection resolution is described based on a structure of single mode -core-only -single mode fiber (SCS) with its multimode (or Mach-Zehnder) interference effect as a filter that is integrated into an erbium-doped fiber laser (EDFL) system for excitation. In the case study, the section of core-only fiber is functionalized with porcine immunoglobulin G (IgG) antibodies, which could selectively bind to bacterial pathogen of Staphylococcus aureus (S. aureus). The developed microfiber-based biosensing platform called SCS-based EDFL biosensors can effectively detect concentrations of S. aureus from 10 to 105 CFU/mL, with a responsivity of 42.6 p.m./(CFU/mL) for the wavelength shift of the measured spectrum. The limit of detection (LoD) is estimated as 7.3 CFU/mL based on the measurement of S. aureus with minimum concentration of 10 CFU/mL. In addition, when a lower concentration of 1 CFU/mL is applied to the biosensor, a wavelength shift of 0.12 nm is observed in 10% of samples (1/10), indicating actual LoD of 1 CFU/mL for the proposed biosensor. Attributed to its good sensitivity, stability, reproducibility and specificity, the proposed EDFL based biosensing platform has great potentials for diagnostics.
KW - Staphylococcus aureus sensor
KW - Foodborne pathogen
KW - Fiber interferometer
KW - Fiber laser biosensor
UR - http://www.scopus.com/inward/record.url?scp=85148545382&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2023.124385
DO - 10.1016/j.talanta.2023.124385
M3 - Article
VL - 257
JO - Talanta
JF - Talanta
SN - 0039-9140
M1 - 124385
ER -