Isothiocyanate-induced Cell Cycle Arrest in a Novel In Vitro Exposure Protocol of Human Malignant Melanoma (A375) Cells

Theodora Mantso; Ioannis Anestopoulos; Eleftheria Lamprianidou; Ioannis Kotsianidis; Aglaia Pappa; Mihalis Panagiotidis

doi:10.21873/anticanres.13152

Isothiocyanate-induced Cell Cycle Arrest in a Novel In Vitro Exposure Protocol of Human Malignant Melanoma (A375) Cells

Theodora Mantso, Ioannis Anestopoulos, Eleftheria Lamprianidou, Ioannis Kotsianidis, Aglaia Pappa, Mihalis Panagiotidis

Applied Sciences Department

Research output: Contribution to journal › Article › peer-review

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Abstract

Background/Aim: Several studies have documented the effects of isothiocyanates (ITCs) on cancer prevention by inducing oxidative stress, activating apoptosis, affecting cell cycle regulation, etc. Previously, we have shown that ITCs, administered at low concentrations by the means of double-bolus are capable of potentiating cytotoxicity in human malignant melanoma (A375) cells by inducing apoptosis. The aim of the present study was to further investigate the effect of the treatment of A375 cells with ITCs on cell cycle progression and the levels of various cell cycle regulators. Materials and Methods: Cell cycle analysis was performed by means of flow cytometry whereas western immunoblotting was used to determine the expression levels of these protein regulators. Results: Our data showed an increase in the number of cells in the G2/M phase accompanied by a decrease in the G0/G1 phase, while several cell-cycle regulators were shown to be differentially expressed upon exposure to ITCs.
Conclusion: ITCs induced cell cycle arrest in A375 cells.

Original language	English
Pages (from-to)	591-596
Journal	Anticancer Research
Volume	39
Issue number	2
DOIs	https://doi.org/10.21873/anticanres.13152
Publication status	Published - 1 Feb 2019

Keywords

Isothiocyanates
malignant melanoma
A375 cells
cell cycle
growth arrest

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/anticanres.13152

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title = "Isothiocyanate-induced Cell Cycle Arrest in a Novel In Vitro Exposure Protocol of Human Malignant Melanoma (A375) Cells",

abstract = "Background/Aim: Several studies have documented the effects of isothiocyanates (ITCs) on cancer prevention by inducing oxidative stress, activating apoptosis, affecting cell cycle regulation, etc. Previously, we have shown that ITCs, administered at low concentrations by the means of double-bolus are capable of potentiating cytotoxicity in human malignant melanoma (A375) cells by inducing apoptosis. The aim of the present study was to further investigate the effect of the treatment of A375 cells with ITCs on cell cycle progression and the levels of various cell cycle regulators. Materials and Methods: Cell cycle analysis was performed by means of flow cytometry whereas western immunoblotting was used to determine the expression levels of these protein regulators. Results: Our data showed an increase in the number of cells in the G2/M phase accompanied by a decrease in the G0/G1 phase, while several cell-cycle regulators were shown to be differentially expressed upon exposure to ITCs. Conclusion: ITCs induced cell cycle arrest in A375 cells.",

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T1 - Isothiocyanate-induced Cell Cycle Arrest in a Novel In Vitro Exposure Protocol of Human Malignant Melanoma (A375) Cells

AU - Mantso, Theodora

AU - Anestopoulos, Ioannis

AU - Lamprianidou, Eleftheria

AU - Kotsianidis, Ioannis

AU - Pappa, Aglaia

AU - Panagiotidis, Mihalis

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Background/Aim: Several studies have documented the effects of isothiocyanates (ITCs) on cancer prevention by inducing oxidative stress, activating apoptosis, affecting cell cycle regulation, etc. Previously, we have shown that ITCs, administered at low concentrations by the means of double-bolus are capable of potentiating cytotoxicity in human malignant melanoma (A375) cells by inducing apoptosis. The aim of the present study was to further investigate the effect of the treatment of A375 cells with ITCs on cell cycle progression and the levels of various cell cycle regulators. Materials and Methods: Cell cycle analysis was performed by means of flow cytometry whereas western immunoblotting was used to determine the expression levels of these protein regulators. Results: Our data showed an increase in the number of cells in the G2/M phase accompanied by a decrease in the G0/G1 phase, while several cell-cycle regulators were shown to be differentially expressed upon exposure to ITCs. Conclusion: ITCs induced cell cycle arrest in A375 cells.

AB - Background/Aim: Several studies have documented the effects of isothiocyanates (ITCs) on cancer prevention by inducing oxidative stress, activating apoptosis, affecting cell cycle regulation, etc. Previously, we have shown that ITCs, administered at low concentrations by the means of double-bolus are capable of potentiating cytotoxicity in human malignant melanoma (A375) cells by inducing apoptosis. The aim of the present study was to further investigate the effect of the treatment of A375 cells with ITCs on cell cycle progression and the levels of various cell cycle regulators. Materials and Methods: Cell cycle analysis was performed by means of flow cytometry whereas western immunoblotting was used to determine the expression levels of these protein regulators. Results: Our data showed an increase in the number of cells in the G2/M phase accompanied by a decrease in the G0/G1 phase, while several cell-cycle regulators were shown to be differentially expressed upon exposure to ITCs. Conclusion: ITCs induced cell cycle arrest in A375 cells.

KW - Isothiocyanates

KW - malignant melanoma

KW - A375 cells

KW - cell cycle

KW - growth arrest

U2 - 10.21873/anticanres.13152

DO - 10.21873/anticanres.13152

M3 - Article

SN - 0250-7005

VL - 39

SP - 591

EP - 596

JO - Anticancer Research

JF - Anticancer Research

IS - 2

ER -

Isothiocyanate-induced Cell Cycle Arrest in a Novel In Vitro Exposure Protocol of Human Malignant Melanoma (A375) Cells

Abstract

Keywords

UN SDGs

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