TY - JOUR
T1 - Large-Scale Fabrication of 3D Scaffold-Based Patterns of Microparticles and Breast Cancer Cells using Reusable Acoustofluidic Device
AU - Dai Nguyen, Tan
AU - Tran, Van-Thai
AU - Pudasaini, Sanam
AU - Gautam, Archana
AU - Lee, Jia Min
AU - Fu, Richard
AU - Du, Hejun
N1 - Funding information: The authors gratefully acknowledge the support of 1) Nanyang Technological University and the Ministry of Education of Singapore through a Ph.D. Scholarship and AcRF Tier 1 research grant (RG 96/18); 2) the UK Engineering and Physical Sciences Research Council (EPSRC) grants (EP/P018998/1); and 3) Special Interesting Group of Acoustofluidics funded by UK Fluids Network (EP/N032861/1).
PY - 2021/6/1
Y1 - 2021/6/1
N2 - Spatial distribution of biological cells plays a key role in tissue engineering for reconstituting the cellular microenvironment, and recently, acoustofluidics are explored as a viable tool for controlling structures in tissue fabrication because of its good biocompatibility, low-power consumption, automation capability, nature of non-invasive, and non-contact. Herein, a reusable acoustofluidic device is developed using surface acoustic waves for manipulating microparticles/cells to form a 3D matrix pattern inside a scaffold-based hydrogel contained in a millimetric chamber. The 3D patterned and polymerized hydrogel construct can be easily and safely removed from the chamber using a proposed lifting technique, which prevent any physical damages or contaminations and promote the reusability of the chamber. The generated 3D patterns of microparticles and cells are numerically studied using a finite-element method, which is well validated by the experimental results. The proposed acoustofluidic device is a useful tool for in vitro engineering 3D scaffold-based artificial tissues for drug and toxicity testing and building organs-on-chip applications.
AB - Spatial distribution of biological cells plays a key role in tissue engineering for reconstituting the cellular microenvironment, and recently, acoustofluidics are explored as a viable tool for controlling structures in tissue fabrication because of its good biocompatibility, low-power consumption, automation capability, nature of non-invasive, and non-contact. Herein, a reusable acoustofluidic device is developed using surface acoustic waves for manipulating microparticles/cells to form a 3D matrix pattern inside a scaffold-based hydrogel contained in a millimetric chamber. The 3D patterned and polymerized hydrogel construct can be easily and safely removed from the chamber using a proposed lifting technique, which prevent any physical damages or contaminations and promote the reusability of the chamber. The generated 3D patterns of microparticles and cells are numerically studied using a finite-element method, which is well validated by the experimental results. The proposed acoustofluidic device is a useful tool for in vitro engineering 3D scaffold-based artificial tissues for drug and toxicity testing and building organs-on-chip applications.
KW - surface acoustic waves
KW - acoustofluidics
KW - microfluidics
KW - 3D patterning
KW - organs-on chips
UR - http://www.scopus.com/inward/record.url?scp=85101939021&partnerID=8YFLogxK
U2 - 10.1002/adem.202001377
DO - 10.1002/adem.202001377
M3 - Article
VL - 23
JO - Advanced Engineering Materials
JF - Advanced Engineering Materials
SN - 1438-1656
IS - 6
M1 - 2001377
ER -