Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: Identification of a novel wax ester

Joanna Bacon; Lynn G. Dover; Kim A. Hatch; Yi Zhang; Jessica M. Gomes; Sharon Kendall; Lorenz Wernisch; Neil G. Stoker; Philip D. Butcher; Gurdyal S. Besra; Philip D. Marsh

doi:10.1099/mic.0.2006/004317-0

Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: Identification of a novel wax ester

Joanna Bacon^*, Lynn G. Dover, Kim A. Hatch, Yi Zhang, Jessica M. Gomes, Sharon Kendall, Lorenz Wernisch, Neil G. Stoker, Philip D. Butcher, Gurdyal S. Besra, Philip D. Marsh

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

The low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. In this study, Mycobacterium tuberculosis H37Rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. A previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menaquinone were also observed between iron-replete and iron-limited chemostat cultures. DNA microarray analysis revealed differential expression of genes involved in glycerolipid metabolism and isoprenoid quinone biosynthesis, providing some insight into the underlying genetic changes that correlate with cell-wall lipid profiles of M. tuberculosis growing in an iron-limited environment.

Original language	English
Pages (from-to)	1435-1444
Number of pages	10
Journal	Microbiology
Volume	153
Issue number	5
DOIs	https://doi.org/10.1099/mic.0.2006/004317-0
Publication status	Published - 1 May 2007
Externally published	Yes

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Bacon, J., Dover, L. G., Hatch, K. A., Zhang, Y., Gomes, J. M., Kendall, S., Wernisch, L., Stoker, N. G., Butcher, P. D., Besra, G. S., & Marsh, P. D. (2007). Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: Identification of a novel wax ester. Microbiology, 153(5), 1435-1444. https://doi.org/10.1099/mic.0.2006/004317-0

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title = "Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: Identification of a novel wax ester",

abstract = "The low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. In this study, Mycobacterium tuberculosis H37Rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. A previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menaquinone were also observed between iron-replete and iron-limited chemostat cultures. DNA microarray analysis revealed differential expression of genes involved in glycerolipid metabolism and isoprenoid quinone biosynthesis, providing some insight into the underlying genetic changes that correlate with cell-wall lipid profiles of M. tuberculosis growing in an iron-limited environment.",

author = "Joanna Bacon and Dover, {Lynn G.} and Hatch, {Kim A.} and Yi Zhang and Gomes, {Jessica M.} and Sharon Kendall and Lorenz Wernisch and Stoker, {Neil G.} and Butcher, {Philip D.} and Besra, {Gurdyal S.} and Marsh, {Philip D.}",

year = "2007",

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doi = "10.1099/mic.0.2006/004317-0",

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Bacon, J, Dover, LG, Hatch, KA, Zhang, Y, Gomes, JM, Kendall, S, Wernisch, L, Stoker, NG, Butcher, PD, Besra, GS & Marsh, PD 2007, 'Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: Identification of a novel wax ester', Microbiology, vol. 153, no. 5, pp. 1435-1444. https://doi.org/10.1099/mic.0.2006/004317-0

TY - JOUR

T1 - Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture

T2 - Identification of a novel wax ester

AU - Bacon, Joanna

AU - Dover, Lynn G.

AU - Hatch, Kim A.

AU - Zhang, Yi

AU - Gomes, Jessica M.

AU - Kendall, Sharon

AU - Wernisch, Lorenz

AU - Stoker, Neil G.

AU - Butcher, Philip D.

AU - Besra, Gurdyal S.

AU - Marsh, Philip D.

PY - 2007/5/1

Y1 - 2007/5/1

N2 - The low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. In this study, Mycobacterium tuberculosis H37Rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. A previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menaquinone were also observed between iron-replete and iron-limited chemostat cultures. DNA microarray analysis revealed differential expression of genes involved in glycerolipid metabolism and isoprenoid quinone biosynthesis, providing some insight into the underlying genetic changes that correlate with cell-wall lipid profiles of M. tuberculosis growing in an iron-limited environment.

AB - The low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. In this study, Mycobacterium tuberculosis H37Rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. A previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menaquinone were also observed between iron-replete and iron-limited chemostat cultures. DNA microarray analysis revealed differential expression of genes involved in glycerolipid metabolism and isoprenoid quinone biosynthesis, providing some insight into the underlying genetic changes that correlate with cell-wall lipid profiles of M. tuberculosis growing in an iron-limited environment.

U2 - 10.1099/mic.0.2006/004317-0

DO - 10.1099/mic.0.2006/004317-0

M3 - Article

C2 - 17464057

AN - SCOPUS:34249066244

SN - 1350-0872

VL - 153

SP - 1435

EP - 1444

JO - Microbiology

JF - Microbiology

IS - 5

ER -

Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: Identification of a novel wax ester

Abstract

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