TY - JOUR
T1 - Localized Plasmon Based Multicore Fiber Biosensor for Acetylcholine Detection
AU - Zhu, Guo
AU - Wang, Yu
AU - Wang, Zhi
AU - Singh, Ragini
AU - Marques, Carlos
AU - Wu, Qiang
AU - Kaushik, Brajesh Kumar
AU - Jha, Rajan
AU - Zhang, Bingyuan
AU - Kumar, Santosh
N1 - Funding information: Research funded by Natural Science Foundation of Shandong Province (ZR2020QC061), Science and Engineering Research Board (TAR/2018/000051), Double Hundred Talent Plan of Shandong Province, Special Construction Project Fund for Shandong Province Taishan Mountain Scholars, Liaocheng University China (31805180301, 318051901, 31805180326). C. Marques acknowledges Fundacao para a Ciencia e a Technologica (FCT) through the CEECIND/00034/2018 (iFish project), UIDB/50025/2020 & UIDP/50025/2020 i£N projects, financed by national funds through the FCT/MEC.
PY - 2022/2/21
Y1 - 2022/2/21
N2 - In this work, tapered/etched multicore fiber (MCF) probes are spliced with multimode fiber (MMF) to fabricate the sensor structure. To improve sensitivity, gold nanoparticles (AuNPs) and molybdenum disulfide nanoparticles (MoS2-NPs) are used to immobilize both probes. Synthesized AuNPs and MoS2-nanoparticles (NPs) have peak absorption wavelengths of 519 nm and 330 nm, respectively. High-resolution transmission electron microscopy is used to examine the morphology of the NPs. The scanning electron microscope is used to characterize the NPs-immobilized optical fiber sensor structures, and SEM-EDX is used to verify the NPs-coating over fiber structure. The functionalization of acetylcholinesterase enzyme over the NPs-immobilized probe increases the specificity of the sensor later on. Finally, the developed sensor probes are tested by detecting various acetylcholine concentrations. In addition, performance analysis such as reusability, reproducibility, and selectivity (in the presence of ascorbic acid, glucose, dopamine, and uric acid) are carried out, and proposed biosensors are experimentally evaluated. The developed tapered fiber sensor with a sensitivity of 0.062 nm/uM can detect even very low concentrations like 14.28 uM over a wide detection range of 0 to 1000 uM.
AB - In this work, tapered/etched multicore fiber (MCF) probes are spliced with multimode fiber (MMF) to fabricate the sensor structure. To improve sensitivity, gold nanoparticles (AuNPs) and molybdenum disulfide nanoparticles (MoS2-NPs) are used to immobilize both probes. Synthesized AuNPs and MoS2-nanoparticles (NPs) have peak absorption wavelengths of 519 nm and 330 nm, respectively. High-resolution transmission electron microscopy is used to examine the morphology of the NPs. The scanning electron microscope is used to characterize the NPs-immobilized optical fiber sensor structures, and SEM-EDX is used to verify the NPs-coating over fiber structure. The functionalization of acetylcholinesterase enzyme over the NPs-immobilized probe increases the specificity of the sensor later on. Finally, the developed sensor probes are tested by detecting various acetylcholine concentrations. In addition, performance analysis such as reusability, reproducibility, and selectivity (in the presence of ascorbic acid, glucose, dopamine, and uric acid) are carried out, and proposed biosensors are experimentally evaluated. The developed tapered fiber sensor with a sensitivity of 0.062 nm/uM can detect even very low concentrations like 14.28 uM over a wide detection range of 0 to 1000 uM.
KW - acetylcholine
KW - etched fiber structure
KW - gold nanoparticles
KW - molybdenum disulfide
KW - Optical fiber sensor
KW - tapered fiber structure
UR - http://www.scopus.com/inward/record.url?scp=85121344062&partnerID=8YFLogxK
U2 - 10.1109/TIM.2021.3133335
DO - 10.1109/TIM.2021.3133335
M3 - Article
AN - SCOPUS:85121344062
SN - 0018-9456
VL - 71
JO - IEEE Transactions on Instrumentation and Measurement
JF - IEEE Transactions on Instrumentation and Measurement
M1 - 7000309
ER -