M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s

Jianquan Li, Nanmao Dang, Nuria Martinez-Lopez, Paul A. Jowsey, Dong Huang, Robert N. Lightowlers, Fei Gao, Jun-Yong Huang

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
26 Downloads (Pure)

Abstract

Background
Drugs such as taxanes, epothilones, and vinca alkaloids are widely used in the treatment of breast, ovarian, and lung cancers but come with major side effects such as neuropathy and loss of neutrophils and as single agents have a lack of efficacy. M2I-1 (MAD2 inhibitor-1) has been shown to disrupt the CDC20-MAD2 interaction, and consequently, the assembly of the mitotic checkpoint complex (MCC).

Results
We report here that M2I-1 can significantly increase the sensitivity of several cancer cell lines to anti-mitotic drugs, with cell death occurring after a prolonged mitotic arrest. In the presence of nocodazole or taxol combined with M2I-1 cell death is triggered by the premature degradation of Cyclin B1, the perturbation of the microtubule network, and an increase in the level of the pro-apoptotic protein MCL-1s combined with a marginal increase in the level of NOXA. The elevated level of MCL-1s and the marginally increased NOXA antagonized the increased level of MCL-1, a pro-survival protein of the Bcl-2 family.

Conclusion
Our results provide some important molecular mechanisms for understanding the relationship between the mitotic checkpoint and programmed cell death and demonstrate that M2I-1 exhibits antitumor activity in the presence of current anti-mitotic drugs such as taxol and nocodazole and has the potential to be developed as an anticancer agent.
Original languageEnglish
Article number5
Number of pages15
JournalCell Division
Volume14
Issue number1
Early online date15 Jun 2019
DOIs
Publication statusPublished - Dec 2019
Externally publishedYes

Keywords

  • M2I-1
  • Cyclin B1
  • MCL-1
  • Nocodazole
  • Taxol
  • Apoptosis

Fingerprint

Dive into the research topics of 'M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s'. Together they form a unique fingerprint.

Cite this