@inbook{2a19fc74b2114fa5ab1f33b8d7c6a3da,
title = "Measuring Synthesis and Degradation of MHC Class I Molecules",
abstract = "Major histocompatibility complex (MHC) class I molecules function to present pathogen derived peptides to cytotoxic T cells and act as ligands for Natural Killer cells, thus alerting the immune system to the presence of invading pathogens. However, some MHC class I molecules, most notably HLA-B27, can be strongly associated with autoimmune diseases. In addition, the MHC class I pathway is a target for numerous viral evasion strategies Understanding not only the antigen presenting functions, but also the biosynthesis and the degradation pathways of MHC class I molecules has therefore become important in determining their role in pathogen and autoimmune related diseases. Here, we describe how using epitope tagged MHC class I molecules can aid in the analysis of MHC class I molecule biosynthesis and degradation as well as complementary studies using conventional conformationally specific antibodies. Coupled together with pharmacological manipulation which can target both biosynthetic and degradative pathways, this offers a powerful tool in analyzing MHC class I molecules.",
keywords = "Electrophoresis, Polyacrylamide Gel, Epitopes/metabolism, Glycosylation, HEK293 Cells, HeLa Cells, Histocompatibility Antigens Class I/biosynthesis, Humans, Immunoblotting, Indicators and Reagents, Proteolysis",
author = "Powis, \{Simon J\} and Antoniou, \{Antony N\}",
year = "2019",
month = may,
day = "31",
doi = "10.1007/978-1-4939-9450-2\_7",
language = "English",
isbn = "9781493994496",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "83--100",
editor = "\{van Endert\}, Peter",
booktitle = "Antigen Processing",
address = "United States",
}