Miniaturized Magnetic Bead-Actuators for Force-Clamp Spectroscopy-Based Single-Molecule Measurements

Luying Feng, Hamdi Torun

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Abstract

Force-clamp spectroscopy can mimic the physiological conditions for the proteins under investigation. In addition, it is a direct way of observing the relationship between bond lifetime and molecular forces. However, traditional force-clamp methods rely on active feedback controllers that can introduce artefacts. In this work, we introduce a new method to enable force-clamp spectroscopy without a need for an active feedback. The method is based on miniaturized magnetic beads offering improved stability. As a case study, we performed force-clamp experiments using biotin-streptavidin molecule pairs with and without active feedback. Our results demonstrate the feasibility of force-clamp experiments without feedback and illustrate the advantages of our method.
Original languageEnglish
Article number112888
Number of pages6
JournalUltramicroscopy
Volume209
Early online date11 Nov 2019
DOIs
Publication statusPublished - 1 Feb 2020

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