Misincorporation by RNA polymerase is a major source of transcription pausing in vivo

Katherine James, Pamela Gamba, Simon J. Cockell, Nikolay Zenkin

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)
12 Downloads (Pure)

Abstract

The transcription error rate estimated from mistakes in end product RNAs is 10−3–10−5. We analyzed the fidelity of nascent RNAs from all actively transcribing elongation complexes (ECs) in Escherichia coli and Saccharomyces cerevisiae and found that 1–3% of all ECs in wild-type cells, and 5–7% of all ECs in cells lacking proofreading factors are, in fact, misincorporated complexes. With the exception of a number of sequence-dependent hotspots, most misincorporations are distributed relatively randomly. Misincorporation at hotspots does not appear to be stimulated by pausing. Since misincorporation leads to a strong pause of transcription due to backtracking, our findings indicate that misincorporation could be a major source of transcriptional pausing and lead to conflicts with other RNA polymerases and replication in bacteria and eukaryotes. This observation implies that physical resolution of misincorporated complexes may be the main function of the proofreading factors Gre and TFIIS. Although misincorporation mechanisms between bacteria and eukaryotes appear to be conserved, the results suggest the existence of a bacteria-specific mechanism(s) for reducing misincorporation in protein-coding regions. The links between transcription fidelity, human disease, and phenotypic variability in genetically-identical cells can be explained by the accumulation of misincorporated complexes, rather than mistakes in mature RNA.
Original languageEnglish
Pages (from-to)1105-1113
Number of pages9
JournalNucleic Acids Research
Volume45
Issue number3
Early online date24 Oct 2016
DOIs
Publication statusPublished - 1 Feb 2017
Externally publishedYes

Fingerprint

Dive into the research topics of 'Misincorporation by RNA polymerase is a major source of transcription pausing in vivo'. Together they form a unique fingerprint.

Cite this