TY - JOUR
T1 - Modified acid polysaccharide derived from Salvia przewalskii with excellent wound healing and enhanced bioactivity
AU - Zhao, Kui
AU - Qian, Cheng
AU - Qi, Luming
AU - Li, Qing
AU - Zhao, Can
AU - Zhang, Jing
AU - Han, Guiqi
AU - Xia, Lina
AU - El-Bahy, Zeinhom M.
AU - Gu, Junwei
AU - Helal, Mohamed H.
AU - Yan, Zhuyun
AU - Guo, Zhanhu
AU - Shi, Zhengjun
N1 - Funding information: This study was supported financially by the National Natural Science Foundation of China (No. 81973416). Thanks to the support of the postdoctoral station of Forestry Engineering of Southwest Forestry University. The authors extend their appreciation to the Deanship of Scientific Research at Northern Border University, Arar, KSA for funding this research work through the project number “NBU-FFR-2024-540-05”.
PY - 2024/4/1
Y1 - 2024/4/1
N2 - Acid polysaccharide was extracted from Salvia przewalskii root powders (PSP), purified by diethylaminoethyl cellulose column (DEAE-52) and molecular sieve (PSP2). PSPm1 was obtained by modifying PSP2 with nitrite and phosphoric acid. The chemical structure of PSP2 and PSPm1 exhibited notable distinctions, primarily due to the absence of arabinose and promotion of glucuronic acid (GlcA). The structure of PSPm1 was deduced through the utilization of 1H, 13C, and 2-D NMR. The main chain was linked by α-D-Galp(1 → 3)-α-Glcp-(1 → fragments and →6)-β-D-Galp fragments, with the presence of →4)-α-D-GlcpA-(1 → 6)-β-D-Galp-(1 → ， → 4)-α-D-GalAp-(1 → 2,4)-α-D-Rhap-(1 → fragments and →6)-α-Glcp-(1 → 2,4)-β-D-Manp-(1 → fragments. PSPm1 exhibited different immunoregulatory bioactivity in vitro, including haemostatic effects indicated by activated clotting time of 55.5 % reduction by the activated clotting time (ACT) test and wound healing function in vivo. PSPm1 also displayed better anti-tumor biological effects than unmodified. The structure-activity dissimilarity between PSP2 and PSPm1 primarily stems from variations in molecular weight (Mw), monosaccharide composition, and branching patterns. The modification of polysaccharides from the extract residues of Chinese medicinal materials may be a new form of drug supplements.
AB - Acid polysaccharide was extracted from Salvia przewalskii root powders (PSP), purified by diethylaminoethyl cellulose column (DEAE-52) and molecular sieve (PSP2). PSPm1 was obtained by modifying PSP2 with nitrite and phosphoric acid. The chemical structure of PSP2 and PSPm1 exhibited notable distinctions, primarily due to the absence of arabinose and promotion of glucuronic acid (GlcA). The structure of PSPm1 was deduced through the utilization of 1H, 13C, and 2-D NMR. The main chain was linked by α-D-Galp(1 → 3)-α-Glcp-(1 → fragments and →6)-β-D-Galp fragments, with the presence of →4)-α-D-GlcpA-(1 → 6)-β-D-Galp-(1 → ， → 4)-α-D-GalAp-(1 → 2,4)-α-D-Rhap-(1 → fragments and →6)-α-Glcp-(1 → 2,4)-β-D-Manp-(1 → fragments. PSPm1 exhibited different immunoregulatory bioactivity in vitro, including haemostatic effects indicated by activated clotting time of 55.5 % reduction by the activated clotting time (ACT) test and wound healing function in vivo. PSPm1 also displayed better anti-tumor biological effects than unmodified. The structure-activity dissimilarity between PSP2 and PSPm1 primarily stems from variations in molecular weight (Mw), monosaccharide composition, and branching patterns. The modification of polysaccharides from the extract residues of Chinese medicinal materials may be a new form of drug supplements.
KW - Wound healing
KW - Polysaccharide modification
KW - Anti-tumor
UR - http://www.scopus.com/inward/record.url?scp=85184568432&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2024.129803
DO - 10.1016/j.ijbiomac.2024.129803
M3 - Article
C2 - 38296147
SN - 0141-8130
VL - 263
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 129803
ER -