Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds

S. Azra Moosvi, Ian McDonald, David Pearce, Donovan Kelly, Ann Wood

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, South Orkney Islands, Antarctica. Genes encoding the mxaF of methanol dehydrogenase, the fdxA for Afipia ferredoxin, the msmA of methanesulfonate monooxygenase, and the 16S rRNA gene of Methylobacterium were detected in all samples tested. The mxaF gene sequences corresponded to those of Hyphomicrobium, Methylobacterium, and Methylomonas. Over 30 pure cultures of methylotrophs were isolated on methanesulfonate, dimethylsulfone, or dimethylsulfide from ten Signy Island lakes. Some were identified from 16S rRNA gene sequences (and morphology) as Hyphomicrobium species, strains of Afipia felis, and a methylotrophic Flavobacterium strain. Antarctic environments thus contain diverse methylotrophic bacteria, growing on various C1-substrates, including C1-sulfur compounds.
Original languageEnglish
Pages (from-to)541-554
JournalSystematic and Applied Microbiology
Volume28
Issue number6
DOIs
Publication statusPublished - 1 Aug 2005

Fingerprint

Dive into the research topics of 'Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds'. Together they form a unique fingerprint.

Cite this