Multi-Omics of Corynebacterium pseudotuberculosis 12CS0282 and an In Silico Reverse Vaccinology Approach Reveal Novel Vaccine and Drug Targets

Jens Möller, Mona Bodenschatz, Vartul Sangal, Joerg Hofmann Drrernat, Andreas Burkovski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Corynebacterium pseudotuberculosis is an important animal pathogen, which is also able to infect humans. An optimal treatment of infections with this pathogen is not available today and consequently, more research is necessary to understand the infection process. Here, we present a combined -omics and bioinformatics approach to characterize C. pseudotuberculosis 12CS0282. The genome sequence of strain 12CS0282 was determined, analyzed in comparison with the available 130 C. pseudotuberculosis sequences and used as a basis for proteome analyses. In a reverse vaccinology approach, putative vaccine and drug targets for 12CS0208 were identified. Mass spectrometry analyses revealed the presence of multiple virulence factors even without host contact. In macrophage interaction studies, C. pseudotuberculosis 12CS0282 was highly resistant against human phagocytes and even multiplied within human THP-1 cells. Taken together, the data indicate a high pathogenic potential of the strain.
Original languageEnglish
Article number39
Number of pages18
JournalProteomes
Volume10
Issue number4
DOIs
Publication statusPublished - 23 Nov 2022

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