Multicomponent organic nanoparticles for fluorescence studies in biological systems

Tom McDonald; Philip Martin; Joseph Patterson; Darren Smith; Marco Giardiello; Marco Marcello; Violaine See; Rachel O'Reilly; Andrew Owen; Steven Rannard

doi:10.1002/adfm.201103059

Multicomponent organic nanoparticles for fluorescence studies in biological systems

Tom McDonald, Philip Martin, Joseph Patterson, Darren Smith, Marco Giardiello, Marco Marcello, Violaine See, Rachel O'Reilly, Andrew Owen, Steven Rannard

Applied Sciences

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

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Abstract

The formation of dual-component organic nanoparticles by a modified emulsion-templated freeze-drying approach leads to aqueous nanosuspensions showing fluorescence (Förster) resonance energy transfer (FRET) from within a distribution of single nanoparticles. The combination of both FRET dyes within dual-component nanoparticles (<200 nm) allows the spatial and physical monitoring of the particles, as the FRET signal is lost on dissolution and breakdown of the nanoparticles. The monitoring of accumulation by Caco-2 cells and macrophages shows very limited internalization within the non-phagocytic cells. Conservation of FRET within the macrophages confirms extensive whole-particle internalization. The cellular permeability through Caco-2 monolayers is also assessed and movement of intact dual-component particles is observed, suggesting a mechanism for enhanced pharmacokinetics in vivo.

Original language	English
Pages (from-to)	2469-2478
Journal	Advanced Functional Materials
Volume	22
Issue number	12
DOIs	https://doi.org/10.1002/adfm.201103059
Publication status	Published - 20 Jun 2012

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Smith D - Output 1 - Multi-component Organic NanoparticlesSubmitted manuscript, 1.68 MB

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title = "Multicomponent organic nanoparticles for fluorescence studies in biological systems",

abstract = "The formation of dual-component organic nanoparticles by a modified emulsion-templated freeze-drying approach leads to aqueous nanosuspensions showing fluorescence (F{\"o}rster) resonance energy transfer (FRET) from within a distribution of single nanoparticles. The combination of both FRET dyes within dual-component nanoparticles (<200 nm) allows the spatial and physical monitoring of the particles, as the FRET signal is lost on dissolution and breakdown of the nanoparticles. The monitoring of accumulation by Caco-2 cells and macrophages shows very limited internalization within the non-phagocytic cells. Conservation of FRET within the macrophages confirms extensive whole-particle internalization. The cellular permeability through Caco-2 monolayers is also assessed and movement of intact dual-component particles is observed, suggesting a mechanism for enhanced pharmacokinetics in vivo.",

keywords = "nanosuspensions, cellular uptake, fluorescence resonance energy transfer (FRET)",

author = "Tom McDonald and Philip Martin and Joseph Patterson and Darren Smith and Marco Giardiello and Marco Marcello and Violaine See and Rachel O'Reilly and Andrew Owen and Steven Rannard",

note = "This is the pre-peer reviewed version of the following article: McDonald, T. O., Martin, P., Patterson, J. P., Smith, D., Giardiello, M., Marcello, M., See, V., O'Reilly, R. K., Owen, A. and Rannard, S. (2012), Multicomponent Organic Nanoparticles for Fluorescence Studies in Biological Systems. Adv. Funct. Mater., 22: 2469–2478 which has been published in final form at http://dx.doi.org/10.1002/adfm.201103059",

year = "2012",

month = jun,

day = "20",

doi = "10.1002/adfm.201103059",

language = "English",

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journal = "Advanced Functional Materials",

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publisher = "Wiley",

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TY - JOUR

T1 - Multicomponent organic nanoparticles for fluorescence studies in biological systems

AU - McDonald, Tom

AU - Martin, Philip

AU - Patterson, Joseph

AU - Smith, Darren

AU - Giardiello, Marco

AU - Marcello, Marco

AU - See, Violaine

AU - O'Reilly, Rachel

AU - Owen, Andrew

AU - Rannard, Steven

N1 - This is the pre-peer reviewed version of the following article: McDonald, T. O., Martin, P., Patterson, J. P., Smith, D., Giardiello, M., Marcello, M., See, V., O'Reilly, R. K., Owen, A. and Rannard, S. (2012), Multicomponent Organic Nanoparticles for Fluorescence Studies in Biological Systems. Adv. Funct. Mater., 22: 2469–2478 which has been published in final form at http://dx.doi.org/10.1002/adfm.201103059

PY - 2012/6/20

Y1 - 2012/6/20

N2 - The formation of dual-component organic nanoparticles by a modified emulsion-templated freeze-drying approach leads to aqueous nanosuspensions showing fluorescence (Förster) resonance energy transfer (FRET) from within a distribution of single nanoparticles. The combination of both FRET dyes within dual-component nanoparticles (<200 nm) allows the spatial and physical monitoring of the particles, as the FRET signal is lost on dissolution and breakdown of the nanoparticles. The monitoring of accumulation by Caco-2 cells and macrophages shows very limited internalization within the non-phagocytic cells. Conservation of FRET within the macrophages confirms extensive whole-particle internalization. The cellular permeability through Caco-2 monolayers is also assessed and movement of intact dual-component particles is observed, suggesting a mechanism for enhanced pharmacokinetics in vivo.

AB - The formation of dual-component organic nanoparticles by a modified emulsion-templated freeze-drying approach leads to aqueous nanosuspensions showing fluorescence (Förster) resonance energy transfer (FRET) from within a distribution of single nanoparticles. The combination of both FRET dyes within dual-component nanoparticles (<200 nm) allows the spatial and physical monitoring of the particles, as the FRET signal is lost on dissolution and breakdown of the nanoparticles. The monitoring of accumulation by Caco-2 cells and macrophages shows very limited internalization within the non-phagocytic cells. Conservation of FRET within the macrophages confirms extensive whole-particle internalization. The cellular permeability through Caco-2 monolayers is also assessed and movement of intact dual-component particles is observed, suggesting a mechanism for enhanced pharmacokinetics in vivo.

KW - nanosuspensions

KW - cellular uptake

KW - fluorescence resonance energy transfer (FRET)

U2 - 10.1002/adfm.201103059

DO - 10.1002/adfm.201103059

M3 - Article

SN - 1616-301X

VL - 22

SP - 2469

EP - 2478

JO - Advanced Functional Materials

JF - Advanced Functional Materials

IS - 12

ER -

Multicomponent organic nanoparticles for fluorescence studies in biological systems

Abstract

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