Nitrogen-doped carbon hollow trunk-like structure as a portable electrochemical sensor for noradrenaline detection in neuronal cells

Mohammed Y. Emran, Mohamed A. Shenashen, Ahmed Elmarakbi, Mahmoud M. Selim, Sherif A. El-Safty*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

To date, the production and development of portable analytical devices for environmental and healthcare applications are rapidly growing. Herein, a portable electrochemical sensor for monitoring of noradrenaline (NA) secreted from living cells using mesoporous carbon-based materials was fabricated. The modification of the interdigitated electrode array (IDA) by nitrogen-doped mesoporous carbon spheres (N-doped MCS) and nitrogen-doped carbon hollow trunk-like structure (N-doped CHT) was used to fabricate the NA sensor. The N-doped CHT surface shows multiple holes distributed with micrometer-sized open holes (1–2 μm) and nanometer-sized thin walls (∼98 nm). The N-doped CHT surface heterogeneity of wrinkled and twisted hollow trunk structures improve the diffusion pathway and the NA molecules loading. The N-doped CHT/IDA showed a highly selective assay for monitoring of NA with high sensitivity (1770 μA/μM × cm2), a low detection limit (0.005 μM), and a wide linear range (0.01–0.3 μM). The N-doped CHT/IDA monitored the NA secreted from PC12 cells under various concentrations of simulation agents (KCl). The designed N-doped CHT/IDA provides a portable NA-sensor assay with facile signaling, good stability, high biocompatibility, in-vitro assay compatibility, and good reproducibility. Therefore, the designed sensor can be used as a portable sensor for NA detection in live cells and can be matched with portable smartphones after further developments.

Original languageEnglish
Article number339380
Number of pages13
JournalAnalytica Chimica Acta
Volume1192
Early online date16 Dec 2021
DOIs
Publication statusPublished - 1 Feb 2022

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