Osteopontin drives KRAS-mutant lung adenocarcinoma

Ioanna Giopanou, Nikolaos I Kanellakis, Anastasios D Giannou, Ioannis Lilis, Antonia Marazioti, Magda Spella, Vassilios Papaleonidopoulos, Davina C M Simoes, Dimitra E Zazara, Theodora Agalioti, Charalampos Moschos, Sophia Magkouta, Ioannis Kalomenidis, Vily Panoutsakopoulou, Anne-sophie Lamort, Georgios T Stathopoulos, Ioannis Psallidas

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
3 Downloads (Pure)

Abstract

Increased expression of osteopontin (SPP1) is associated with aggressive human lung adenocarcinoma, but its function remains unknown. Our aim was to determine the role of SPP1 in smoking-induced lung adenocarcinoma. We combined mouse models of tobacco carcinogen-induced lung adenocarcinoma, of deficiency of endogenous Spp1 alleles, and of adoptive pulmonary macrophage reconstitution to map the expression of SPP1 and its
receptors and determine its impact during carcinogenesis. Co-expression of Spp1 and mutant KrasG12C in benign cells was employed to investigate SPP1/KRAS interactions in oncogenesis. Finally, intratracheal adenovirus encoding Cre recombinase was delivered to LSL.KRASG12D mice lacking endogenous or overexpressing transgenic Spp1 alleles. SPP1 was overexpressed in experimental and human lung adenocarcinoma and portended poor survival.
In response to two different smoke carcinogens, Spp1-deficient mice developed fewer and smaller lung adenocarcinoma with decreased cellular survival and angiogenesis. Both lung epithelial- and macrophage-secreted SPP1 drove tumor-associated inflammation, while epithelial SPP1 promoted early tumorigenesis by fostering the survival of KRAS-mutated cells. Finally, loss and overexpression of Spp1 was, respectively, protective and deleterious
for mice harboring KRASG12D-driven LADC. Our data support that SPP1 is functionally involved in early stages of airway epithelial carcinogenesis driven by smoking and mutant KRAS and may present an important therapeutic target.
Original languageEnglish
Pages (from-to)1134-1144
JournalCarcinogenesis
Volume41
Issue number8
Early online date19 Nov 2019
DOIs
Publication statusPublished - 1 Aug 2020

Fingerprint Dive into the research topics of 'Osteopontin drives KRAS-mutant lung adenocarcinoma'. Together they form a unique fingerprint.

Cite this