TY - JOUR
T1 - Pathways of propionate degradation by enriched methanogenic cultures
AU - Koch, M.
AU - Dolfing, J.
AU - Wuhrmann, K.
AU - Zehnder, A. J.B.
PY - 1983/4/1
Y1 - 1983/4/1
N2 - A mixed methanogenic culture was highly enriched in a growth medium containing propionate as the sole organic carbon and energy source. With this culture, the pathways of propionate degradation were studied by use of 14C-radiotracers. Propionate was first metabolized to acetate, carbon dioxide, and hydrogen by nonmethanogenic organisms. Formate was not excreted. The carbon dioxide originated exclusively from the carboxyl group of propionate, whereas both [2-14C]- and [3-14C]propionate lead to the production of radioactive acetate. The methyl and carboxyl groups of the acetate produced were equally labeled, regardless of whether [2-14C]- or [3-14C]propionate was used. These observations suggest that in the culture, propionate was degraded through a randomizing pathway.
AB - A mixed methanogenic culture was highly enriched in a growth medium containing propionate as the sole organic carbon and energy source. With this culture, the pathways of propionate degradation were studied by use of 14C-radiotracers. Propionate was first metabolized to acetate, carbon dioxide, and hydrogen by nonmethanogenic organisms. Formate was not excreted. The carbon dioxide originated exclusively from the carboxyl group of propionate, whereas both [2-14C]- and [3-14C]propionate lead to the production of radioactive acetate. The methyl and carboxyl groups of the acetate produced were equally labeled, regardless of whether [2-14C]- or [3-14C]propionate was used. These observations suggest that in the culture, propionate was degraded through a randomizing pathway.
UR - http://www.scopus.com/inward/record.url?scp=0020569681&partnerID=8YFLogxK
U2 - 10.1128/aem.45.4.1411-1414.1983
DO - 10.1128/aem.45.4.1411-1414.1983
M3 - Article
AN - SCOPUS:0020569681
SN - 0099-2240
VL - 45
SP - 1411
EP - 1414
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 4
ER -