TY - JOUR
T1 - Phyllostachys nigra (Lodd. ex Lindl.) derived polysaccharide with enhanced glycolipid metabolism regulation and mice gut microbiome
AU - Zhao, Kui
AU - Wu, Xueyi
AU - Han, Guiqi
AU - Sun, Lin
AU - Zheng, Changwen
AU - Hou, Hua
AU - Xu, Ben Bin
AU - El-Bahy, Zeinhom M.
AU - Qian, Cheng
AU - Kallel, Mohamed
AU - Algadi, Hassan
AU - Guo, Zhanhu
AU - Shi, Zhengjun
N1 - Funding information: There was financial support provided by the National Natural Science Foundation of China (Nos. 31971741, 31760195). Research for thisstudy was also supported by Yibin’s municipal science and technology plan project for the bamboo industry (No. 412-2022PY15). The authors extend their appreciation to the Deanship of Scientific Research at Northern Border University, Arar, KSA for funding this research work through the project number “NBU-FFR-2023-0165".
PY - 2024/2/1
Y1 - 2024/2/1
N2 - This study focuses on the characterization and regulation of glycolipid metabolism of polysaccharides derived from biomass of Phyllostachys nigra (Lodd. ex Lindl.) root (PNr). The extracts from dilute hydrochloric acid, hot water, and 2 % sodium hydroxide solution were characterized through molecular weight, gel permeation chromatography, monosaccharides, Fourier transform infrared, and nuclear magnetic resonance spectroscopy analyses. Polysaccharide from alkali extraction and molecular sieve purification (named as: PNS2A) exhibited optimal inhibitory of 3T3-L1 cellular differentiation and lowered insulin resistance. The PNS2A is made of a hemicellulose-like main chain of →4)-β-D-Xylp-(1→ that was connected by branches of 4-O-Me-α-GlcAp-(1→, T-α-D-Galp-(1→, T-α-L-Araf-(1→, →2)-α-L-Araf-(1→, as well as β-D-Glcp-(1→4-β-D-Glcp-(1→ fragments. Oral delivery of PNS2A in diabetes mice brought down blood glucose and cholesterol levels and regulated glucose and lipid metabolism. PNS2A alleviated diabetes symptoms and body weight and protected liver and kidney function in model animals by altering the gut microbiome. Polysaccharides can be a new approach to develop bamboo resources.
AB - This study focuses on the characterization and regulation of glycolipid metabolism of polysaccharides derived from biomass of Phyllostachys nigra (Lodd. ex Lindl.) root (PNr). The extracts from dilute hydrochloric acid, hot water, and 2 % sodium hydroxide solution were characterized through molecular weight, gel permeation chromatography, monosaccharides, Fourier transform infrared, and nuclear magnetic resonance spectroscopy analyses. Polysaccharide from alkali extraction and molecular sieve purification (named as: PNS2A) exhibited optimal inhibitory of 3T3-L1 cellular differentiation and lowered insulin resistance. The PNS2A is made of a hemicellulose-like main chain of →4)-β-D-Xylp-(1→ that was connected by branches of 4-O-Me-α-GlcAp-(1→, T-α-D-Galp-(1→, T-α-L-Araf-(1→, →2)-α-L-Araf-(1→, as well as β-D-Glcp-(1→4-β-D-Glcp-(1→ fragments. Oral delivery of PNS2A in diabetes mice brought down blood glucose and cholesterol levels and regulated glucose and lipid metabolism. PNS2A alleviated diabetes symptoms and body weight and protected liver and kidney function in model animals by altering the gut microbiome. Polysaccharides can be a new approach to develop bamboo resources.
KW - Phyllostachys nigra
KW - Polysaccharides
KW - Type 2 diabetes
KW - Glycolipid metabolism
KW - Gut microbiome
UR - http://www.scopus.com/inward/record.url?scp=85178499941&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.128588
DO - 10.1016/j.ijbiomac.2023.128588
M3 - Article
SN - 0141-8130
VL - 257
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - Part 1
M1 - 128588
ER -
