Phyllostachys nigra (Lodd. ex Lindl.) derived polysaccharide with enhanced glycolipid metabolism regulation and mice gut microbiome

Kui Zhao, Xueyi Wu, Guiqi Han, Lin Sun, Changwen Zheng, Hua Hou, Ben Bin Xu, Zeinhom M. El-Bahy, Cheng Qian, Mohamed Kallel, Hassan Algadi, Zhanhu Guo*, Zhengjun Shi*

*Corresponding author for this work

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Abstract

This study focuses on the characterization and regulation of glycolipid metabolism of polysaccharides derived from biomass of Phyllostachys nigra (Lodd. ex Lindl.) root (PNr). The extracts from dilute hydrochloric acid, hot water, and 2 % sodium hydroxide solution were characterized through molecular weight, gel permeation chromatography, monosaccharides, Fourier transform infrared, and nuclear magnetic resonance spectroscopy analyses. Polysaccharide from alkali extraction and molecular sieve purification (named as: PNS2A) exhibited optimal inhibitory of 3T3-L1 cellular differentiation and lowered insulin resistance. The PNS2A is made of a hemicellulose-like main chain of →4)-β-D-Xylp-(1→ that was connected by branches of 4-O-Me-α-GlcAp-(1→, T-α-D-Galp-(1→, T-α-L-Araf-(1→, →2)-α-L-Araf-(1→, as well as β-D-Glcp-(1→4-β-D-Glcp-(1→ fragments. Oral delivery of PNS2A in diabetes mice brought down blood glucose and cholesterol levels and regulated glucose and lipid metabolism. PNS2A alleviated diabetes symptoms and body weight and protected liver and kidney function in model animals by altering the gut microbiome. Polysaccharides can be a new approach to develop bamboo resources.
Original languageEnglish
Article number128588
Number of pages12
JournalInternational Journal of Biological Macromolecules
Volume257
Issue numberPart 1
Early online date2 Dec 2023
DOIs
Publication statusPublished - 1 Feb 2024

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