Prevalence and molecular characterization of Enterobacteriaceae producing NDM-1 carbapenemase at a military hospital in Pakistan and evaluation of two chromogenic media

Kathryn Day, Shamshad Ali, Irfin Ali Mirza, Hanna Sidjabat, Anna Silvey, Clare Lanyon, Stephen Cummings, Shahid Abbasi, Muhammad Raza, David Paterson, John Perry

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The aim of this study was to assess the frequency and genotypic diversity of carbapenemase-producing Enterobacteriaceae (CPE) in stool samples from patients attending a military hospital in Pakistan. Further aims included the identification of factors that might predispose to faecal carriage and evaluation of 2 chromogenic culture media: Brilliance CRE and chromID CARBA. Of 175 patients, 32 (18.3%) had faecal carriage of CPE and all produced NDM-1 carbapenemase. All of these 32 patients were detected using chromID CARBA compared with 20 patients (62.5%) detected using Brilliance CRE (P = 0.0015). Duration of hospitalization and treatment with coamoxyclav were statistically associated with a higher likelihood of carriage of CPE (P ≤ 0.05). Themajority of NDM-1–producing Enterobacteriaceae co-produced CTX-M-1 group extended spectrum β-lactamase, and one third produced armA-type methylase. NDM-1 carbapenemase was most commonly found amongst commensal types of Escherichia coli, especially phylogenetic group B1.
Original languageEnglish
Pages (from-to)187-191
JournalDiagnostic Microbiology and Infectious Disease
Volume75
Issue number2
DOIs
Publication statusPublished - Feb 2013

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