Prevalence of NDM-1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media

K. M. Day; M. Salman; B. Kazi; H. E. Sidjabat; A. Silvey; C. V. Lanyon; S. P. Cummings; M. N. Ali; M. W. Raza; D. L. Paterson; John D. Perry

doi:10.1111/jam.12171

Prevalence of NDM-1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media

K. M. Day, M. Salman, B. Kazi, H. E. Sidjabat, A. Silvey, C. V. Lanyon, S. P. Cummings, M. N. Ali, M. W. Raza, D. L. Paterson, John D. Perry^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Aims: To evaluate two chromogenic media, Brilliance CRE and chromID CARBA, with stool samples referred to the Public Health Laboratories Division of the National Institute of Health in Islamabad, and assess the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in this population. Methods and Results: One hundred and fifty-two stool samples from patients with diarrhoea were referred to the Microbiology Department and were investigated for the presence of CPE using two chromogenic culture media, Brilliance CRE and chromID CARBA. Thirteen patients (8·6%) were found to be colonized with CPE and all produced NDM-1 carbapenemase. Twelve of these patients (92%) were found to be colonized by culture on chromID CARBA compared with seven (54%) using Brilliance CRE. Conclusions: If only coloured colonies were considered as presumptive CPE, the sensitivity, specificity and positive predictive value were 54, 23 and 6% for Brilliance CRE and 85, 85 and 36% for chromID CARBA, respectively. Significance and Impact of the Study: We conclude that Enterobacteriaceae that produce NDM-1 carbapenemase can be found in patients from all major provinces of Pakistan and that chromID CARBA was the most effective of the two chromogenic media in this setting.

Original language	English
Pages (from-to)	1810-1816
Number of pages	7
Journal	Journal of Applied Microbiology
Volume	114
Issue number	6
Early online date	19 Feb 2013
DOIs	https://doi.org/10.1111/jam.12171
Publication status	Published - 1 Jun 2013

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@article{9173ad8dadb644528943fe01b5c1164b,

title = "Prevalence of NDM-1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media",

abstract = "Aims: To evaluate two chromogenic media, Brilliance CRE and chromID CARBA, with stool samples referred to the Public Health Laboratories Division of the National Institute of Health in Islamabad, and assess the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in this population. Methods and Results: One hundred and fifty-two stool samples from patients with diarrhoea were referred to the Microbiology Department and were investigated for the presence of CPE using two chromogenic culture media, Brilliance CRE and chromID CARBA. Thirteen patients (8·6%) were found to be colonized with CPE and all produced NDM-1 carbapenemase. Twelve of these patients (92%) were found to be colonized by culture on chromID CARBA compared with seven (54%) using Brilliance CRE. Conclusions: If only coloured colonies were considered as presumptive CPE, the sensitivity, specificity and positive predictive value were 54, 23 and 6% for Brilliance CRE and 85, 85 and 36% for chromID CARBA, respectively. Significance and Impact of the Study: We conclude that Enterobacteriaceae that produce NDM-1 carbapenemase can be found in patients from all major provinces of Pakistan and that chromID CARBA was the most effective of the two chromogenic media in this setting.",

author = "Day, {K. M.} and M. Salman and B. Kazi and Sidjabat, {H. E.} and A. Silvey and Lanyon, {C. V.} and Cummings, {S. P.} and Ali, {M. N.} and Raza, {M. W.} and Paterson, {D. L.} and Perry, {John D.}",

year = "2013",

month = jun,

day = "1",

doi = "10.1111/jam.12171",

language = "English",

volume = "114",

pages = "1810--1816",

journal = "Journal of Applied Microbiology",

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TY - JOUR

T1 - Prevalence of NDM-1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media

AU - Day, K. M.

AU - Salman, M.

AU - Kazi, B.

AU - Sidjabat, H. E.

AU - Silvey, A.

AU - Lanyon, C. V.

AU - Cummings, S. P.

AU - Ali, M. N.

AU - Raza, M. W.

AU - Paterson, D. L.

AU - Perry, John D.

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Aims: To evaluate two chromogenic media, Brilliance CRE and chromID CARBA, with stool samples referred to the Public Health Laboratories Division of the National Institute of Health in Islamabad, and assess the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in this population. Methods and Results: One hundred and fifty-two stool samples from patients with diarrhoea were referred to the Microbiology Department and were investigated for the presence of CPE using two chromogenic culture media, Brilliance CRE and chromID CARBA. Thirteen patients (8·6%) were found to be colonized with CPE and all produced NDM-1 carbapenemase. Twelve of these patients (92%) were found to be colonized by culture on chromID CARBA compared with seven (54%) using Brilliance CRE. Conclusions: If only coloured colonies were considered as presumptive CPE, the sensitivity, specificity and positive predictive value were 54, 23 and 6% for Brilliance CRE and 85, 85 and 36% for chromID CARBA, respectively. Significance and Impact of the Study: We conclude that Enterobacteriaceae that produce NDM-1 carbapenemase can be found in patients from all major provinces of Pakistan and that chromID CARBA was the most effective of the two chromogenic media in this setting.

AB - Aims: To evaluate two chromogenic media, Brilliance CRE and chromID CARBA, with stool samples referred to the Public Health Laboratories Division of the National Institute of Health in Islamabad, and assess the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in this population. Methods and Results: One hundred and fifty-two stool samples from patients with diarrhoea were referred to the Microbiology Department and were investigated for the presence of CPE using two chromogenic culture media, Brilliance CRE and chromID CARBA. Thirteen patients (8·6%) were found to be colonized with CPE and all produced NDM-1 carbapenemase. Twelve of these patients (92%) were found to be colonized by culture on chromID CARBA compared with seven (54%) using Brilliance CRE. Conclusions: If only coloured colonies were considered as presumptive CPE, the sensitivity, specificity and positive predictive value were 54, 23 and 6% for Brilliance CRE and 85, 85 and 36% for chromID CARBA, respectively. Significance and Impact of the Study: We conclude that Enterobacteriaceae that produce NDM-1 carbapenemase can be found in patients from all major provinces of Pakistan and that chromID CARBA was the most effective of the two chromogenic media in this setting.

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DO - 10.1111/jam.12171

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SN - 1364-5072

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JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

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ER -

Prevalence of NDM-1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media

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