TY - JOUR
T1 - Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models
AU - Karapetsas, Athanasios
AU - Voulgaridou, Georgia-persephoni
AU - Konialis, Manolis
AU - Tsochantaridis, Ilias
AU - Kynigopoulos, Spyridon
AU - Lambropoulou, Maria
AU - Stavropoulou, Maria-ioanna
AU - Stathopoulou, Konstantina
AU - Aligiannis, Nektarios
AU - Bozidis, Petros
AU - Goussia, Anna
AU - Gardikis, Konstantinos
AU - Panayiotidis, Mihalis I.
AU - Pappa, Aglaia
PY - 2019/5/9
Y1 - 2019/5/9
N2 - The aim of this study was to assess the antioxidant, photoprotective, and antiaging effects of Greek propolis. Propolis was subjected to n-heptane or methanol extraction. Total phenolic/flavonoid content and antioxidant potential were determined in the extracts. Promising extracts were evaluated for their cytoprotective properties using human immortalized keratinocyte (HaCaT) or reconstituted human skin tissue following exposure to UVB. Assessment of cytotoxicity, DNA damage, oxidative status, and gene/protein expression levels of various matrix metalloproteinases (MMPs) were performed. The propolis methanolic fractions exhibited higher total phenolic and flavonoid contents and significant in vitro antioxidant activity. Incubation of HaCaT cells with certain methanolic extracts significantly decreased the formation of DNA strand breaks following exposure to UVB and attenuated UVB-induced decrease in cell viability. The extracts had no remarkable effect on the total antioxidant status, but significantly lowered total protein carbonyl content used as a marker for protein oxidation in HaCaT cells. MMP-1, -3, -7, and -9, monitored as endpoints of antiaging efficacy, were significantly reduced by propolis following UVB exposure in a model of reconstituted skin tissue. In conclusion, propolis protects against the oxidative and photodamaging effects of UVB and could be further explored as a promising agent for developing natural antiaging strategies.
AB - The aim of this study was to assess the antioxidant, photoprotective, and antiaging effects of Greek propolis. Propolis was subjected to n-heptane or methanol extraction. Total phenolic/flavonoid content and antioxidant potential were determined in the extracts. Promising extracts were evaluated for their cytoprotective properties using human immortalized keratinocyte (HaCaT) or reconstituted human skin tissue following exposure to UVB. Assessment of cytotoxicity, DNA damage, oxidative status, and gene/protein expression levels of various matrix metalloproteinases (MMPs) were performed. The propolis methanolic fractions exhibited higher total phenolic and flavonoid contents and significant in vitro antioxidant activity. Incubation of HaCaT cells with certain methanolic extracts significantly decreased the formation of DNA strand breaks following exposure to UVB and attenuated UVB-induced decrease in cell viability. The extracts had no remarkable effect on the total antioxidant status, but significantly lowered total protein carbonyl content used as a marker for protein oxidation in HaCaT cells. MMP-1, -3, -7, and -9, monitored as endpoints of antiaging efficacy, were significantly reduced by propolis following UVB exposure in a model of reconstituted skin tissue. In conclusion, propolis protects against the oxidative and photodamaging effects of UVB and could be further explored as a promising agent for developing natural antiaging strategies.
KW - propolis
KW - antioxidant
KW - antiaging
KW - photoprotective
KW - DNA damage
KW - matrix metalloproteinases
KW - HaCaT
KW - reconstituted skin
U2 - 10.3390/antiox8050125
DO - 10.3390/antiox8050125
M3 - Article
SN - 2076-3921
VL - 8
JO - Antioxidants
JF - Antioxidants
IS - 5
M1 - 125
ER -