TY - JOUR
T1 - Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biology
AU - Khan, Shahid M.
AU - Franke-Fayard, Blandine
AU - Mair, Gunnar R.
AU - Lasonder, Edwin
AU - Janse, Chris J.
AU - Mann, Matthias
AU - Waters, Andrew P.
N1 - Funding information: The authors wish to thank G. McFadden and G. van Dooren for annotated lists of Plasmodium mitochondrial and apicoplast proteins. M. van Dijk is thanked for providing parasite clones in advance of publication. We would also like to thank R. van der Linden and M. van der Keur for all their help and expertise in using the flow cytometry equipment and the CEBI group for all their assistance and helpful discussions. In particular, we would like to gratefully acknowledge J. Ramesar for all his technical support. This work was supported by the Dutch Science Foundation (NWO/Genomics grant number 050-10-053), the Wellcome Trust Functional Genomics Initiative, Leiden University Medical Centre, Stimuleerings Fonds, and the Danish National Research Foundation.
PY - 2005/6/3
Y1 - 2005/6/3
N2 - Gametocytes, the precursor cells of malaria-parasite gametes, circulate in the blood and are responsible for transmission from host to mosquito vector. The individual proteomes of male and female gametocytes were analyzed using mass spectrometry, following separation by flow sorting of transgenic parasites expressing green fluorescent protein, in a sex-specific manner. Promoter tagging in transgenic parasites confirmed the designation of stage and sex specificity of the proteins. The male proteome contained 36% (236 of 650) male-specific and the female proteome 19% (101 of 541) female-specific proteins, but they share only 69 proteins, emphasizing the diverged features of the sexes. Of all the malaria life-cycle stages analyzed, the male gametocyte has the most distinct proteome, containing many proteins involved in flagellar-based motility and rapid genome replication. By identification of gender-specific protein kinases and phosphatases and using targeted gene disruption of two kinases, new sex-specific regulatory pathways were defined.
AB - Gametocytes, the precursor cells of malaria-parasite gametes, circulate in the blood and are responsible for transmission from host to mosquito vector. The individual proteomes of male and female gametocytes were analyzed using mass spectrometry, following separation by flow sorting of transgenic parasites expressing green fluorescent protein, in a sex-specific manner. Promoter tagging in transgenic parasites confirmed the designation of stage and sex specificity of the proteins. The male proteome contained 36% (236 of 650) male-specific and the female proteome 19% (101 of 541) female-specific proteins, but they share only 69 proteins, emphasizing the diverged features of the sexes. Of all the malaria life-cycle stages analyzed, the male gametocyte has the most distinct proteome, containing many proteins involved in flagellar-based motility and rapid genome replication. By identification of gender-specific protein kinases and phosphatases and using targeted gene disruption of two kinases, new sex-specific regulatory pathways were defined.
UR - http://www.scopus.com/inward/record.url?scp=20444405371&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2005.03.027
DO - 10.1016/j.cell.2005.03.027
M3 - Article
C2 - 15935755
AN - SCOPUS:20444405371
SN - 0092-8674
VL - 121
SP - 675
EP - 687
JO - Cell
JF - Cell
IS - 5
ER -
