TY - JOUR
T1 - Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity
AU - Lasonder, Edwin
AU - Janse, Chris J.
AU - Van Gemert, Geert Jan
AU - Mair, Gunnar R.
AU - Vermunt, Adriaan M.W.
AU - Douradinha, Bruno G.
AU - Van Noort, Vera
AU - Huynen, Martijn A.
AU - Luty, Adrian J.F.
AU - Kroeze, Hans
AU - Khan, Shahid M.
AU - Sauerwein, Robert W.
AU - Waters, Andrew P.
AU - Mann, Matthias
AU - Stunnenberg, Hendrik G.
N1 - Funding information: Part of this work was supported by the Dutch Science Foundation (NWO/Genomics, grant number 050-10-053) and The Wellcome Trust Functional Genomics Initiative. EL and SMK were supported by a programme from the Dutch NWO/Genomics, AMWV and GJvG by Maltrans (EU Framework Programme V), GRM was supported by BioMalPar and is a recipient of a Netherlands Genomics Initiative HORIZON Project Grant (050-71-061), and SMK is supported by TI-Pharma (T4-102). This study was supported by the WHO.
PY - 2008/10/31
Y1 - 2008/10/31
N2 - Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito - early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.
AB - Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito - early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.
UR - http://www.scopus.com/inward/record.url?scp=55449113575&partnerID=8YFLogxK
U2 - 10.1371/journal.ppat.1000195
DO - 10.1371/journal.ppat.1000195
M3 - Article
C2 - 18974882
AN - SCOPUS:55449113575
SN - 1553-7366
VL - 4
JO - PLoS Pathogens
JF - PLoS Pathogens
IS - 10
M1 - e1000195
ER -
