TY - JOUR
T1 - Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function
AU - Worah, Kuntal
AU - Mathan, Till S.M.
AU - Vu Manh, Thien Phong
AU - Keerthikumar, Shivakumar
AU - Schreibelt, Gerty
AU - Tel, Jurjen
AU - Duiveman-de Boer, Tjitske
AU - Sköld, Annette E.
AU - van Spriel, Annemiek B.
AU - de Vries, I. Jolanda M.
AU - Huynen, Martijn A.
AU - Wessels, Hans J.
AU - Gloerich, Jolein
AU - Dalod, Marc
AU - Lasonder, Edwin
AU - Figdor, Carl G.
AU - Buschow, Sonja I.
PY - 2016/9/13
Y1 - 2016/9/13
N2 - Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomic data with existing mRNA data to derive robust cell-specific expression signatures with more than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1β secretion in response to ATP.
AB - Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomic data with existing mRNA data to derive robust cell-specific expression signatures with more than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1β secretion in response to ATP.
UR - http://www.scopus.com/inward/record.url?scp=84991594455&partnerID=8YFLogxK
UR - https://pearl.plymouth.ac.uk/handle/10026.1/5448
U2 - 10.1016/j.celrep.2016.08.023
DO - 10.1016/j.celrep.2016.08.023
M3 - Article
C2 - 27626665
AN - SCOPUS:84991594455
SN - 2211-1247
VL - 16
SP - 2953
EP - 2966
JO - Cell Reports
JF - Cell Reports
IS - 11
ER -