Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function

Kuntal Worah, Till S.M. Mathan, Thien Phong Vu Manh, Shivakumar Keerthikumar, Gerty Schreibelt, Jurjen Tel, Tjitske Duiveman-de Boer, Annette E. Sköld, Annemiek B. van Spriel, I. Jolanda M. de Vries, Martijn A. Huynen, Hans J. Wessels, Jolein Gloerich, Marc Dalod, Edwin Lasonder, Carl G. Figdor*, Sonja I. Buschow

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)
3 Downloads (Pure)

Abstract

Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomic data with existing mRNA data to derive robust cell-specific expression signatures with more than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1β secretion in response to ATP.

Original languageEnglish
Pages (from-to)2953-2966
Number of pages14
JournalCell Reports
Volume16
Issue number11
Early online date13 Sep 2016
DOIs
Publication statusPublished - 13 Sep 2016
Externally publishedYes

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