Abstract
Leukaemia niche impacts quiescence, however culturing patient derived samples ex vivo is technically challenging. Here, we present a protocol for in vitro co-culture of patient-derived-xenograft acute lymphoblastic leukaemia (PDX-ALL) cells with human mesenchymal stem cells (MSC). We describe steps for labeling PDX-ALL cells with CellTrace™ Violet dye to demonstrate MSC-primed PDX-ALL cycling. We then detail procedures to identify MSC-primed G0/quiescent PDX-ALL cells via Hoechst-33342 / Pyronin Y live cell cycle analysis.
Original language | English |
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Article number | 103202 |
Number of pages | 18 |
Journal | STAR Protocols |
Volume | 5 |
Issue number | 3 |
Early online date | 20 Jul 2024 |
DOIs | |
Publication status | Published - 20 Sept 2024 |
Keywords
- Cell Differentiation
- Cell-based Assays
- Stem Cells