Purification of astroglial-cell cultures from rat spinal cord: the use of d-valine to inhibit fibroblast growth

A. J. Cholewinski; J. C. Reid; Alison McDermott; G. P. Wilkin

doi:10.1016/0197-0186(89)90146-0

Purification of astroglial-cell cultures from rat spinal cord: the use of d-valine to inhibit fibroblast growth

A. J. Cholewinski, J. C. Reid, Alison McDermott, G. P. Wilkin

Applied Sciences Department

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Primary astrocyte cultures from spinal cord were purified from contaminating fibroblasts by growth in Dulbecco's modified Eagle's medium (DMEM) in which l-valine was substituted by d-valine. This medium was not supportive for growth of fibroblasts and inhibited their proliferation. The culture purity was assessed using immunofluorescence labelling with specific antibodies to various cell markers. Cultures contained predominantly astrocytes with greater than 92% of this cell type in d-valine medium as opposed to approx. 70% in d,l-valine DMEM medium. This procedure enables primary cultures to be obtained with a larger percentage of astrocytes by a simple modification to the growth medium.

Original language	English
Pages (from-to)	365-369
Journal	Neurochemistry International
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/0197-0186(89)90146-0
Publication status	Published - 1989

Access to Document

10.1016/0197-0186(89)90146-0

https://doi.org/10.1016/0197-0186(89)90146-0

Cite this

@article{31e69f97728a41489a583e36dc0401d1,

title = "Purification of astroglial-cell cultures from rat spinal cord: the use of d-valine to inhibit fibroblast growth",

abstract = "Primary astrocyte cultures from spinal cord were purified from contaminating fibroblasts by growth in Dulbecco's modified Eagle's medium (DMEM) in which l-valine was substituted by d-valine. This medium was not supportive for growth of fibroblasts and inhibited their proliferation. The culture purity was assessed using immunofluorescence labelling with specific antibodies to various cell markers. Cultures contained predominantly astrocytes with greater than 92% of this cell type in d-valine medium as opposed to approx. 70% in d,l-valine DMEM medium. This procedure enables primary cultures to be obtained with a larger percentage of astrocytes by a simple modification to the growth medium.",

author = "Cholewinski, {A. J.} and Reid, {J. C.} and Alison McDermott and Wilkin, {G. P.}",

year = "1989",

doi = "10.1016/0197-0186(89)90146-0",

language = "English",

volume = "15",

pages = "365--369",

journal = "Neurochemistry International",

issn = "0197-0186",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Purification of astroglial-cell cultures from rat spinal cord: the use of d-valine to inhibit fibroblast growth

AU - Cholewinski, A. J.

AU - Reid, J. C.

AU - McDermott, Alison

AU - Wilkin, G. P.

PY - 1989

Y1 - 1989

N2 - Primary astrocyte cultures from spinal cord were purified from contaminating fibroblasts by growth in Dulbecco's modified Eagle's medium (DMEM) in which l-valine was substituted by d-valine. This medium was not supportive for growth of fibroblasts and inhibited their proliferation. The culture purity was assessed using immunofluorescence labelling with specific antibodies to various cell markers. Cultures contained predominantly astrocytes with greater than 92% of this cell type in d-valine medium as opposed to approx. 70% in d,l-valine DMEM medium. This procedure enables primary cultures to be obtained with a larger percentage of astrocytes by a simple modification to the growth medium.

AB - Primary astrocyte cultures from spinal cord were purified from contaminating fibroblasts by growth in Dulbecco's modified Eagle's medium (DMEM) in which l-valine was substituted by d-valine. This medium was not supportive for growth of fibroblasts and inhibited their proliferation. The culture purity was assessed using immunofluorescence labelling with specific antibodies to various cell markers. Cultures contained predominantly astrocytes with greater than 92% of this cell type in d-valine medium as opposed to approx. 70% in d,l-valine DMEM medium. This procedure enables primary cultures to be obtained with a larger percentage of astrocytes by a simple modification to the growth medium.

U2 - 10.1016/0197-0186(89)90146-0

DO - 10.1016/0197-0186(89)90146-0

M3 - Article

SN - 0197-0186

VL - 15

SP - 365

EP - 369

JO - Neurochemistry International

JF - Neurochemistry International

IS - 3

ER -

Purification of astroglial-cell cultures from rat spinal cord: the use of d-valine to inhibit fibroblast growth

Abstract

Access to Document

Fingerprint

Cite this