Reliability of relaxation properties of knee-extensor muscles induced by transcranial magnetic stimulation

Gianluca Vernillo*, Chiara Barbi, John Temesi, Gaia Giurato, Fabio Giuseppe Laginestra, Camilla Martignon, Federico Schena, Massimo Venturelli

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
16 Downloads (Pure)

Abstract

Transcranial magnetic stimulation (TMS)-induced relaxation rate reflects intrinsic muscle contractile properties by interrupting the drive from the central nervous system during voluntary muscle contractions. To determine the appropriateness of knee-extensor muscle relaxation measurements induced by TMS, this study aimed to establish both the within- and between-session reliability before and after a fatiguing exercise bout. Eighteen participants (9 females, 9 males, age 25 ± 2 years, height 171 ± 9 cm, body mass 68.5 ± 13.5 kg) volunteered to participate in two identical sessions approximately 30 days apart. Maximal and submaximal neuromuscular evaluations were performed with TMS six times before (PRE) and at the end (POST) of a 2-min sustained maximal voluntary isometric contraction. Within- and between-session reliability of PRE values were assessed with intraclass correlation coefficient (ICC2,1, relative reliability), repeatability coefficient (absolute reliability), and coefficient of variation (variability). Test-retest reliability of post-exercise muscle relaxation rates was assessed with Bland-Altman plots. For both the absolute and normalized peak relaxation rates and time to peak relaxation, data demonstrated low variability (e.g. coefficient of variation ≤ 7.8%) and high reliability (e.g. ICC2,1 ≥ 0.963). Bland-Altman plots showed low systematic errors. These findings establish the reliability of TMS-induced muscle relaxation rates in unfatigued and fatigued knee-extensor muscles, showing that TMS is a useful technique that researchers can use when investigating changes in muscle relaxation rates both in unfatigued and fatigued knee-extensor muscles.
Original languageEnglish
Article number136694
JournalNeuroscience Letters
Volume782
Early online date21 May 2022
DOIs
Publication statusPublished - 1 Jun 2022

Keywords

  • fatigue
  • knee extensors
  • reliability
  • transcranial magnetic stimulation
  • muscle relaxation rate

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