Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites

Melissa van Dijk; Rinke Vinkenoog; Jai Ramesar; Richard Vervenne; Andrew Waters; Chris Janse

doi:10.1016/S0166-6851(97)02843-0

Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites

Melissa van Dijk, Rinke Vinkenoog, Jai Ramesar, Richard Vervenne, Andrew Waters, Chris Janse

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

To fully exploit the transfection technology developed for Plasmodium we investigated the features of replication, expression and segregation of an episomally maintained DNA construct during asexual blood stage development in genetically transformed parasites of P. berghei. Using DNA in situ hybridisation techniques we were able to show that the introduced DNA construct is located in the nucleus of the parasite and is not segregating uniformly during schizogony. Replication of the construct mainly takes place between 16 and 24 h after invasion of the merozoites, coinciding with chromosomal replication. Furthermore the plasmid-borne DHFR/TS gene is constitutively transcribed throughout the asexual blood stage development. Hence the DHFR/TS promoter would appear to be a useful tool in the study of (over)expression of introduced genes and performing complementation studies in transfected parasites during the complete asexual blood stage development of P. berghei.

Original language	English
Pages (from-to)	155-162
Journal	Molecular and Biochemical Parasitology
Volume	86
Issue number	2
DOIs	https://doi.org/10.1016/S0166-6851(97)02843-0
Publication status	Published - Jun 1997

Keywords

Plasmodium
Transfection
Episomal
Transcription
Replication
Segregation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0166-6851(97)02843-0

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title = "Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites",

abstract = "To fully exploit the transfection technology developed for Plasmodium we investigated the features of replication, expression and segregation of an episomally maintained DNA construct during asexual blood stage development in genetically transformed parasites of P. berghei. Using DNA in situ hybridisation techniques we were able to show that the introduced DNA construct is located in the nucleus of the parasite and is not segregating uniformly during schizogony. Replication of the construct mainly takes place between 16 and 24 h after invasion of the merozoites, coinciding with chromosomal replication. Furthermore the plasmid-borne DHFR/TS gene is constitutively transcribed throughout the asexual blood stage development. Hence the DHFR/TS promoter would appear to be a useful tool in the study of (over)expression of introduced genes and performing complementation studies in transfected parasites during the complete asexual blood stage development of P. berghei.",

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T1 - Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites

AU - van Dijk, Melissa

AU - Vinkenoog, Rinke

AU - Ramesar, Jai

AU - Vervenne, Richard

AU - Waters, Andrew

AU - Janse, Chris

PY - 1997/6

Y1 - 1997/6

N2 - To fully exploit the transfection technology developed for Plasmodium we investigated the features of replication, expression and segregation of an episomally maintained DNA construct during asexual blood stage development in genetically transformed parasites of P. berghei. Using DNA in situ hybridisation techniques we were able to show that the introduced DNA construct is located in the nucleus of the parasite and is not segregating uniformly during schizogony. Replication of the construct mainly takes place between 16 and 24 h after invasion of the merozoites, coinciding with chromosomal replication. Furthermore the plasmid-borne DHFR/TS gene is constitutively transcribed throughout the asexual blood stage development. Hence the DHFR/TS promoter would appear to be a useful tool in the study of (over)expression of introduced genes and performing complementation studies in transfected parasites during the complete asexual blood stage development of P. berghei.

AB - To fully exploit the transfection technology developed for Plasmodium we investigated the features of replication, expression and segregation of an episomally maintained DNA construct during asexual blood stage development in genetically transformed parasites of P. berghei. Using DNA in situ hybridisation techniques we were able to show that the introduced DNA construct is located in the nucleus of the parasite and is not segregating uniformly during schizogony. Replication of the construct mainly takes place between 16 and 24 h after invasion of the merozoites, coinciding with chromosomal replication. Furthermore the plasmid-borne DHFR/TS gene is constitutively transcribed throughout the asexual blood stage development. Hence the DHFR/TS promoter would appear to be a useful tool in the study of (over)expression of introduced genes and performing complementation studies in transfected parasites during the complete asexual blood stage development of P. berghei.

KW - Plasmodium

KW - Transfection

KW - Episomal

KW - Transcription

KW - Replication

KW - Segregation

UR - https://www.scopus.com/pages/publications/0030985978

U2 - 10.1016/S0166-6851(97)02843-0

DO - 10.1016/S0166-6851(97)02843-0

M3 - Article

SN - 0166-6851

VL - 86

SP - 155

EP - 162

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 2

ER -

Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites

Abstract

Keywords

UN SDGs

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