TY - CHAP
T1 - Revolutionizing Prokaryotic Systematics Through Next-Generation Sequencing
AU - Sangal, Vartul
AU - Nieminen, Leena
AU - Tucker, Nicholas
AU - Hoskisson, Paul
PY - 2014
Y1 - 2014
N2 - Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.
AB - Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.
KW - mapping
KW - SNP analyses
KW - phylogenetic analyses
KW - prokaryotic systematics
UR - https://www.scopus.com/pages/publications/84919419857
U2 - 10.1016/bs.mim.2014.07.001
DO - 10.1016/bs.mim.2014.07.001
M3 - Chapter
SN - 9780128001769
VL - 41
T3 - Methods in Microbiology
SP - 75
EP - 101
BT - New Approaches to Prokaryotic Systematics
PB - Elsevier
CY - London
ER -