@article{7289b4f394494818b4f85eb34a63ed3a,
title = "Rhythmic potassium transport regulates the circadian clock in human red blood cells",
abstract = "Circadian rhythms organize many aspects of cell biology and physiology to a daily temporal program that depends on clock gene expression cycles in most mammalian cell types. However, circadian rhythms are also observed in isolated mammalian red blood cells (RBCs), which lack nuclei, suggesting the existence of post-translational cellular clock mechanisms in these cells. Here we show using electrophysiological and pharmacological approaches that human RBCs display circadian regulation of membrane conductance and cytoplasmic conductivity that depends on the cycling of cytoplasmic K+ levels. Using pharmacological intervention and ion replacement, we show that inhibition of K+ transport abolishes RBC electrophysiological rhythms. Our results suggest that in the absence of conventional transcription cycles, RBCs maintain a circadian rhythm in membrane electrophysiology through dynamic regulation of K+ transport.",
author = "Henslee, {Erin A.} and Priya Crosby and Kitcatt, {Stephen J.} and Parry, {Jack S.W.} and Andrea Bernardini and Abdallat, {Rula G.} and Gabriella Braun and Fatoyinbo, {Henry O.} and Harrison, {Esther J.} and Edgar, {Rachel S.} and Hoettges, {Kai F.} and Reddy, {Akhilesh B.} and Jabr, {Rita I.} and {Von Schantz}, Malcolm and O'Neill, {John S.} and Labeed, {Fatima H.}",
note = "We are grateful to Jo Westmoreland of Visual Aids for graphical design, the Biomedical Services Group for animal care, the donors and Noel Wardell for assistance with the blood samples, Michael Hastings and Elizabeth Maywood for supplying mouse tissue and for useful discussion, which was also provided by Brandon Reeder, Daan R. van der Veen, Michael P. Hughes. We also thank Gill Wallis for training on FACS. Finally we thank Jason Day for ICP-MS analysis. FHL and MvS were supported by a subcontract from an Engineering and Physical Research Council-EPSRC grant (EP/1000992/1); JON is supported by the Medical Research Council (MC_UP_1201/4) and the Wellcome Trust (093734/Z/10/Z). ABR is supported by The Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001534), the UK Medical Research Council (FC001534), and the Wellcome Trust (FC001534). This research was also supported by a Biotechnology and Biological Research Council-BBSRC grant to FHL, MvS, RIJ, JON, and ABR (BB/M021556/1).",
year = "2017",
month = dec,
doi = "10.1038/s41467-017-02161-4",
language = "English",
volume = "8",
pages = "1--10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}