TY - JOUR
T1 - Role of physical and chemical interactions in the antibacterial behavior of ZnO nanoparticles against E. coli
AU - Jiang, Yunhong
AU - Zhang, Lingling
AU - Wen, Dongsheng
AU - Ding, Yulong
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Zinc oxide (ZnO) nanoparticles (NPs) exhibit antibacterial activity against both Gram-positive and Gram-negative bacteria. However, the antimicrobial mechanism of ZnO NPs remains unclear. In this study, we investigated the interactions among ZnO NPs, released chemicals (Zn2 + and Reactive Oxygen Species, ROS) and Escherichia coli (E. coli) cells. ZnO NPs without contacting with bacterial cells showed strong antibacterial effect. The results of the leakage of intracellular K+ and integrity of carboxyfluoresce in-filled liposomes showed that ZnO NPs have antimicrobial activity against E. coli by non-specifically disrupting E. coli membranes. Traces of zinc ions (1.25 mg/L) and hydrogen peroxide (from 1.25 to 4.5 μM/L) were detected in ZnO NPs suspensions, but was insufficient to cause the antibacterial effect. However, the addition of radical scavengers suppressed the bactericidal effect of ZnO coated films against E. coli, potentially implicating ROS generation, especially hydroxyl radicals, in the antibacterial ability of ZnO NPs.
AB - Zinc oxide (ZnO) nanoparticles (NPs) exhibit antibacterial activity against both Gram-positive and Gram-negative bacteria. However, the antimicrobial mechanism of ZnO NPs remains unclear. In this study, we investigated the interactions among ZnO NPs, released chemicals (Zn2 + and Reactive Oxygen Species, ROS) and Escherichia coli (E. coli) cells. ZnO NPs without contacting with bacterial cells showed strong antibacterial effect. The results of the leakage of intracellular K+ and integrity of carboxyfluoresce in-filled liposomes showed that ZnO NPs have antimicrobial activity against E. coli by non-specifically disrupting E. coli membranes. Traces of zinc ions (1.25 mg/L) and hydrogen peroxide (from 1.25 to 4.5 μM/L) were detected in ZnO NPs suspensions, but was insufficient to cause the antibacterial effect. However, the addition of radical scavengers suppressed the bactericidal effect of ZnO coated films against E. coli, potentially implicating ROS generation, especially hydroxyl radicals, in the antibacterial ability of ZnO NPs.
KW - Antibacterial mechanism
KW - Bio-interaction
KW - E. coli
KW - ZnO NPs
UR - http://www.scopus.com/inward/record.url?scp=84989808964&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2016.08.044
DO - 10.1016/j.msec.2016.08.044
M3 - Article
C2 - 27612837
AN - SCOPUS:84989808964
SN - 0928-4931
VL - 69
SP - 1361
EP - 1366
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -