Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide

Chan Ju Wang; Nicola Laurieri; Areej Abuhammad; Edward Lowe; Isaac Westwood; Ali Ryan; Edith Sim

doi:10.1107/S1744309109044741

Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide

Chan Ju Wang, Nicola Laurieri, Areej Abuhammad, Edward Lowe, Isaac Westwood, Ali Ryan, Edith Sim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Azoreductase 1 from Pseudomonas aeruginosa strain PAO1 (paAzoR1) catalyses the activation of the prodrug balsalazide and reduces the azo dye methyl red using reduced nicotinamide adenine dinucleotide cofactor as an electron donor. To investigate the mechanism of the enzyme, a Y131F mutation was introduced and the enzymic properties of the mutant were compared with those of the wild-type enzyme. The crystallographic structure of the mutant with methyl red bound was solved at 2.1 Å resolution and compared with the wild-type structure. Tyr131 is important in the architecture of the active site but is not essential for enzymic activity.

Original language	English
Pages (from-to)	2-7
Number of pages	6
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	66
Issue number	1
DOIs	https://doi.org/10.1107/S1744309109044741
Publication status	Published - 25 Dec 2009
Externally published	Yes

Access to Document

10.1107/S1744309109044741

Cite this

Wang, C. J., Laurieri, N., Abuhammad, A., Lowe, E., Westwood, I., Ryan, A., & Sim, E. (2009). Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(1), 2-7. https://doi.org/10.1107/S1744309109044741

@article{088e62e7ded6440badf9a6f3a8003a89,

title = "Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide",

abstract = "Azoreductase 1 from Pseudomonas aeruginosa strain PAO1 (paAzoR1) catalyses the activation of the prodrug balsalazide and reduces the azo dye methyl red using reduced nicotinamide adenine dinucleotide cofactor as an electron donor. To investigate the mechanism of the enzyme, a Y131F mutation was introduced and the enzymic properties of the mutant were compared with those of the wild-type enzyme. The crystallographic structure of the mutant with methyl red bound was solved at 2.1 {\AA} resolution and compared with the wild-type structure. Tyr131 is important in the architecture of the active site but is not essential for enzymic activity.",

author = "Wang, {Chan Ju} and Nicola Laurieri and Areej Abuhammad and Edward Lowe and Isaac Westwood and Ali Ryan and Edith Sim",

year = "2009",

month = dec,

day = "25",

doi = "10.1107/S1744309109044741",

language = "English",

volume = "66",

pages = "2--7",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "Blackwell Publishing",

number = "1",

}

Wang, CJ, Laurieri, N, Abuhammad, A, Lowe, E, Westwood, I, Ryan, A & Sim, E 2009, 'Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 66, no. 1, pp. 2-7. https://doi.org/10.1107/S1744309109044741

Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide. / Wang, Chan Ju; Laurieri, Nicola; Abuhammad, Areej et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 1, 25.12.2009, p. 2-7.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide

AU - Wang, Chan Ju

AU - Laurieri, Nicola

AU - Abuhammad, Areej

AU - Lowe, Edward

AU - Westwood, Isaac

AU - Ryan, Ali

AU - Sim, Edith

PY - 2009/12/25

Y1 - 2009/12/25

N2 - Azoreductase 1 from Pseudomonas aeruginosa strain PAO1 (paAzoR1) catalyses the activation of the prodrug balsalazide and reduces the azo dye methyl red using reduced nicotinamide adenine dinucleotide cofactor as an electron donor. To investigate the mechanism of the enzyme, a Y131F mutation was introduced and the enzymic properties of the mutant were compared with those of the wild-type enzyme. The crystallographic structure of the mutant with methyl red bound was solved at 2.1 Å resolution and compared with the wild-type structure. Tyr131 is important in the architecture of the active site but is not essential for enzymic activity.

AB - Azoreductase 1 from Pseudomonas aeruginosa strain PAO1 (paAzoR1) catalyses the activation of the prodrug balsalazide and reduces the azo dye methyl red using reduced nicotinamide adenine dinucleotide cofactor as an electron donor. To investigate the mechanism of the enzyme, a Y131F mutation was introduced and the enzymic properties of the mutant were compared with those of the wild-type enzyme. The crystallographic structure of the mutant with methyl red bound was solved at 2.1 Å resolution and compared with the wild-type structure. Tyr131 is important in the architecture of the active site but is not essential for enzymic activity.

U2 - 10.1107/S1744309109044741

DO - 10.1107/S1744309109044741

M3 - Article

AN - SCOPUS:75149171792

SN - 1744-3091

VL - 66

SP - 2

EP - 7

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 1

ER -

Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide

Abstract

Access to Document

Fingerprint

Cite this